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Literature summary for 1.1.2.8 extracted from

  • Kanchanarach, W.; Theeragool, G.; Yakushi, T.; Toyama, H.; Adachi, O.; Matsushita, K.
    Characterization of thermotolerant Acetobacter pasteurianus strains and their quinoprotein alcohol dehydrogenases (2010), Appl. Microbiol. Biotechnol., 85, 741-751.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene adhA, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs Acetobacter pasteurianus
gene adhB, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs Acetobacter pasteurianus
gene adhS, DNA and amino acid sequence determination and analysis, sequence comparison of the genes encoding subunit I, subunit II, and subunit III of ADHs Acetobacter pasteurianus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ethanol + 2 ferricytochrome c Acetobacter pasteurianus
-
ethanal + 2 ferrocytochrome c
-
?
ethanol + 2 ferricytochrome c Acetobacter pasteurianus MSU10
-
ethanal + 2 ferrocytochrome c
-
?
ethanol + 2 ferricytochrome c Acetobacter pasteurianus IFO3191
-
ethanal + 2 ferrocytochrome c
-
?
ethanol + 2 ferricytochrome c Acetobacter pasteurianus SKU1108
-
ethanal + 2 ferrocytochrome c
-
?

Organic Solvent Stability

Organic Solvent Comment Organism
acetic acid ADH from strain IFO3191 retains 36% of the activities after treating with 2% acetic acid at 30┬░C for 30 min, and 45% after incubation with 4% acetic acid at 25┬░C for 30 min, unstable in 4% acetic acid at 30┬░C Acetobacter pasteurianus
acetic acid ADH from strain MSU10 shows the highest resistance to acetic acid. The enzyme retains over 90% of the original activity after 30 min incubation with 2% acetic acid at 30┬░C and also with 4% acetic acid at 25┬░C, unstable in 4% acetic acid at 30┬░C Acetobacter pasteurianus
acetic acid ADH from strain SKU1108 retains 61% of the activities after treating with 2% acetic acid at 30┬░C for 30 min, and 79% after incubation with 4% acetic acid at 25┬░C for 30 min, unstable in 4% acetic acid at 30┬░C Acetobacter pasteurianus

Organism

Organism UniProt Comment Textmining
Acetobacter pasteurianus C7G3B7 gene adhA encoding subunit I; isolated from passion fruit
-
Acetobacter pasteurianus C7G3B8 gene adhB encoding subunit II; isolated from passion fruit
-
Acetobacter pasteurianus C9K501 gene adhA encoding subunit I
-
Acetobacter pasteurianus C9K502 gene adhB encoding subunit II
-
Acetobacter pasteurianus C9K504 gene adhS encoding subunit III
-
Acetobacter pasteurianus C9W9E3 gene adhS encoding the subunit III
-
Acetobacter pasteurianus D2SZY4 gene adhA encoding the large subunit
-
Acetobacter pasteurianus D2SZY5 gene adhB encoding the cytochrome c subunit
-
Acetobacter pasteurianus IFO3191 C9K501 gene adhA encoding subunit I
-
Acetobacter pasteurianus IFO3191 C9K502 gene adhB encoding subunit II
-
Acetobacter pasteurianus IFO3191 C9K504 gene adhS encoding subunit III
-
Acetobacter pasteurianus MSU10 C7G3B7 gene adhA encoding subunit I; isolated from passion fruit
-
Acetobacter pasteurianus MSU10 C7G3B8 gene adhB encoding subunit II; isolated from passion fruit
-
Acetobacter pasteurianus SKU1108 C9W9E3 gene adhS encoding the subunit III
-
Acetobacter pasteurianus SKU1108 D2SZY4 gene adhA encoding the large subunit
-
Acetobacter pasteurianus SKU1108 D2SZY5 gene adhB encoding the cytochrome c subunit
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme from strain IFO3191 by ultracentrifugation and anion exchange chromatography Acetobacter pasteurianus
native enzyme from strain MSU10 by ultracentrifugation and anion exchange chromatography Acetobacter pasteurianus
native enzyme from strain SKU1108 by ultracentrifugation and anion exchange chromatography Acetobacter pasteurianus

Source Tissue

Source Tissue Comment Organism Textmining
additional information the MSU10 strain shows higher acetic acid productivity in a medium containing 6% ethanol at 37┬░C than strain SKU1108, while strain SKU1108 strain can accumulate more acetic acid in a medium supplemented with 4-5% ethanol at the same temperature. The fermentation ability at 37┬░C of these thermotolerant strains is superior to that of mesophilic strain IFO3191 having weak growth and very delayed acetic acid production at 37┬░C even at 4% ethanol Acetobacter pasteurianus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ethanol + 2 ferricytochrome c
-
Acetobacter pasteurianus ethanal + 2 ferrocytochrome c
-
?
ethanol + 2 ferricytochrome c
-
Acetobacter pasteurianus MSU10 ethanal + 2 ferrocytochrome c
-
?
ethanol + 2 ferricytochrome c
-
Acetobacter pasteurianus IFO3191 ethanal + 2 ferrocytochrome c
-
?
ethanol + 2 ferricytochrome c
-
Acetobacter pasteurianus SKU1108 ethanal + 2 ferrocytochrome c
-
?

Synonyms

Synonyms Comment Organism
quinoprotein alcohol dehydrogenase
-
Acetobacter pasteurianus

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Acetobacter pasteurianus
35
-
-
Acetobacter pasteurianus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
assay at Acetobacter pasteurianus

Cofactor

Cofactor Comment Organism Structure
pyrroloquinoline quinone
-
Acetobacter pasteurianus

General Information

General Information Comment Organism
physiological function the ADH involved in ethanol oxidation of the thermotolerant strain is important for the high temperature fermentation Acetobacter pasteurianus