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Literature summary for 1.1.2.7 extracted from

  • Goodwin, M.G.; Avezoux, A.; Dales, S.L.; Anthony, C.
    Reconstitution of the quinoprotein methanol dehydrogenase from inactive Ca(2+)-free enzyme with Ca2+, Sr2+ or Ba2+ (1996), Biochem. J., 319, 839-842.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
additional information construction of Ca2+-lacking, catalytically inactive mutant apoenzymes, reconstitution of the active holoenzyme by incorporation of two exogenous Ca2+ into the active sites of the alpha-subunits of the alpha2beta2 tetramer Methylorubrum extorquens

Localization

Localization Comment Organism GeneOntology No. Textmining
periplasm
-
Methylorubrum extorquens
-
-
soluble
-
Methylorubrum extorquens
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ba2+ Ca2+ can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+, Ba2+ shows 102% of the activity with Ca2+ Methylorubrum extorquens
Ca2+ 2 Ca2+ ions are irreversibly incorporated per alpha2beta2 tetramer. Calcium can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+ Methylorubrum extorquens
Sr2+ Ca2+ can be replaced in the incorporation process by strontium or barium, the affinities for these ions being similar to that for Ca2+, Sr2+ shows 94% of the activity with Ca2+ Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
methanol + ferricytochrome cL Methylorubrum extorquens
-
formaldehyde + ferrocytochrome cL
-
?

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens P16027 and P14775 P16027 (large subunit, alpha) and P14775 (small subunit, beta); gene mxaA
-

Renatured (Commentary)

Renatured (Comment) Organism
reconstitution of the active holoenzyme by incorporation of two exogenous Ca2+ into the active sites of the alpha-subunits of the alpha2beta2 tetramer, time course of Ca2+ incorporation, overview Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
methanol + ferricytochrome cL
-
Methylorubrum extorquens formaldehyde + ferrocytochrome cL
-
?

Synonyms

Synonyms Comment Organism
quinoprotein methanol dehydrogenase
-
Methylorubrum extorquens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Methylorubrum extorquens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
10
-
about Methylorubrum extorquens

pH Range

pH Minimum pH Maximum Comment Organism
7 10.5 pH profile, overview Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
cytochrome cL electron acceptor Methylorubrum extorquens
pyrroloquinoline quinone PQQ, prosthetic group Methylorubrum extorquens