Cloned (Comment) | Organism |
---|---|
recombinant PVADH tagged with hexahistidine is expressed in Escherichia coli. The three genes encoding oxidized polyvinyl alcohol hydrolase (EC 3.7.1.7), poly(vinyl alcohol) dehydrogenase and cytochrome c are expressed constitutively and form an operon | Sphingopyxis sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
periplasm | - |
Sphingopyxis sp. | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sphingopyxis sp. | Q588Z1 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant enzyme | Sphingopyxis sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
18 | - |
- |
Sphingopyxis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
polyvinyl alcohol + oxidized 2,6-dichlorophenolindophenol | polyvinyl alcohol(117) | Sphingopyxis sp. | polyvinyl alcohol containing oxo-groups + reduced 2,6-dichlorophenolindophenol | - |
? |
Synonyms | Comment | Organism |
---|---|---|
poly(vinyl alcohol) dehydrogenase | - |
Sphingopyxis sp. |
PVA dehydrogenase | - |
Sphingopyxis sp. |
pvaA | - |
Sphingopyxis sp. |
PVADH | - |
Sphingopyxis sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Sphingopyxis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Sphingopyxis sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
pyrroloquinoline quinone | - |
Sphingopyxis sp. |