BRENDA - Enzyme Database
show all sequences of 1.1.2.5

D-Lactate dehydrogenase of Desulfovibrio vulgaris

Ogata, M.; Arihara, K.; Yagi, T.; J. Biochem. 89, 1423-1431 (1981)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
EDTA
slight stimulation
Desulfovibrio vulgaris
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Desulfovibrio vulgaris
cyanide
-
Desulfovibrio vulgaris
PCMB
-
Desulfovibrio vulgaris
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
D-lactate
-
Desulfovibrio vulgaris
2.4
-
DL-2-hydroxybutyrate
-
Desulfovibrio vulgaris
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-lactate + ferricytochrome c-553
Desulfovibrio vulgaris
starting enzyme for carbon and energy metabolism
pyruvate + ferrocytochrome c-553
-
-
?
Organism
Organism
UniProt
Commentary
Textmining
Desulfovibrio vulgaris
-
-
-
Purification (Commentary)
Purification (Commentary)
Organism
partially
Desulfovibrio vulgaris
Storage Stability
Storage Stability
Organism
-20°C, 2 days, 20% loss of activity
Desulfovibrio vulgaris
4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activity
Desulfovibrio vulgaris
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
D-lactate + 1-methoxyphenazinium methyl sulfate
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
D-lactate + 2,6-dichlorophenolindophenol
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
D-lactate + ferricyanide
-
389630
Desulfovibrio vulgaris
pyruvate + ferrocyanide
-
-
-
?
D-lactate + ferricytochrome c-553
-
389630
Desulfovibrio vulgaris
pyruvate + ferrocytochrome c-553
-
389630
Desulfovibrio vulgaris
?
D-lactate + ferricytochrome c-553
starting enzyme for carbon and energy metabolism
389630
Desulfovibrio vulgaris
pyruvate + ferrocytochrome c-553
-
-
-
?
D-lactate + methylene blue
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
D-lactate + nitrotetrazolium blue
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
DL-2-hydroxybutyrate + ferricytochrome c-553
-
389630
Desulfovibrio vulgaris
2-ketobutyrate + ferrocytochrome c-553
-
389630
Desulfovibrio vulgaris
?
additional information
no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity
389630
Desulfovibrio vulgaris
?
-
-
-
?
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
D-lactate + nitrotetrazolium blue
Desulfovibrio vulgaris
pH Range
pH Minimum
pH Maximum
Commentary
Organism
7
9
pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue
Desulfovibrio vulgaris
Cofactor
Cofactor
Commentary
Organism
Structure
Ferricytochrome c-553
-
Desulfovibrio vulgaris
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
EDTA
slight stimulation
Desulfovibrio vulgaris
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
Ferricytochrome c-553
-
Desulfovibrio vulgaris
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
-
Desulfovibrio vulgaris
cyanide
-
Desulfovibrio vulgaris
PCMB
-
Desulfovibrio vulgaris
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.8
-
D-lactate
-
Desulfovibrio vulgaris
2.4
-
DL-2-hydroxybutyrate
-
Desulfovibrio vulgaris
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
D-lactate + ferricytochrome c-553
Desulfovibrio vulgaris
starting enzyme for carbon and energy metabolism
pyruvate + ferrocytochrome c-553
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
partially
Desulfovibrio vulgaris
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, 2 days, 20% loss of activity
Desulfovibrio vulgaris
4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activity
Desulfovibrio vulgaris
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
D-lactate + 1-methoxyphenazinium methyl sulfate
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
D-lactate + 2,6-dichlorophenolindophenol
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
D-lactate + ferricyanide
-
389630
Desulfovibrio vulgaris
pyruvate + ferrocyanide
-
-
-
?
D-lactate + ferricytochrome c-553
-
389630
Desulfovibrio vulgaris
pyruvate + ferrocytochrome c-553
-
389630
Desulfovibrio vulgaris
?
D-lactate + ferricytochrome c-553
starting enzyme for carbon and energy metabolism
389630
Desulfovibrio vulgaris
pyruvate + ferrocytochrome c-553
-
-
-
?
D-lactate + methylene blue
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
D-lactate + nitrotetrazolium blue
-
389630
Desulfovibrio vulgaris
pyruvate + ?
-
-
-
?
DL-2-hydroxybutyrate + ferricytochrome c-553
-
389630
Desulfovibrio vulgaris
2-ketobutyrate + ferrocytochrome c-553
-
389630
Desulfovibrio vulgaris
?
additional information
no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity
389630
Desulfovibrio vulgaris
?
-
-
-
?
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
8
-
D-lactate + nitrotetrazolium blue
Desulfovibrio vulgaris
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
7
9
pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue
Desulfovibrio vulgaris
Other publictions for EC 1.1.2.5
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
389630
Ogata
D-Lactate dehydrogenase of Des ...
Desulfovibrio vulgaris
J. Biochem.
89
1423-1431
1981
1
-
-
-
-
-
3
2
-
-
-
1
-
6
-
-
1
-
-
-
-
2
10
-
-
-
-
-
-
1
1
-
1
-
-
-
1
-
-
1
-
-
-
-
3
-
2
-
-
-
1
-
-
-
1
-
-
-
2
10
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-