Cloned (Comment) | Organism |
---|---|
into vector pET16 and expressed in Escherichia coli BLR DE3 pLysS | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mRNAs are present in both d2hgdh mutant lines, but not in dldh1-1 and dldh1-2 mutant lines | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.061 | - |
D-2-hydroxybutyrate | - |
Arabidopsis thaliana | |
0.164 | - |
D-lactate | - |
Arabidopsis thaliana | |
0.432 | - |
glycolate | - |
Arabidopsis thaliana | |
4.486 | - |
L-lactate | - |
Arabidopsis thaliana | |
8.871 | - |
D-glycerate | - |
Arabidopsis thaliana |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
59000 | - |
2 * 59000, sequence analysis | Arabidopsis thaliana |
135000 | - |
gel filtration | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q94AX4 | - |
- |
Purification (Comment) | Organism |
---|---|
by centrifugation, sonication and on Ni2+-NTA column, to near homogeneity | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-2-hydroxybutyrate + ferricytochrome c | 135% relative activity compared to (R)lactate, highest catalytic efficiency, prefered substrate | Arabidopsis thaliana | ? | - |
? | |
D-2-hydroxyglutarate + ferricytochrome c | 0.15% relative activity compared to (R)lactate | Arabidopsis thaliana | ? | - |
? | |
D-glycerate + ferricytochrome c | 9.2% relative activity compared to (R)lactate | Arabidopsis thaliana | ? | - |
? | |
D-lactate + 2 ferricytochrome c | 100% relative activity, prefered substrate, with a high catalytic efficiency 200- and 2000fold higher than that for L-lactate and glycolate, respectively | Arabidopsis thaliana | pyruvate + 2 ferrocytochrome c | - |
? | |
DL-glycerate + ferricytochrome c | 10.3% relative activity compared to (R)lactate | Arabidopsis thaliana | ? | - |
? | |
glycolate + ferricytochrome c | 0.15% relative activity compared to (R)lactate, O2 does not function as the acceptor for glycolate electrons | Arabidopsis thaliana | ? | - |
? | |
L-lactate + ferricytochrome c | 10.8% relative activity compared to (R)lactate | Arabidopsis thaliana | ? | - |
? | |
additional information | no activity with propionate, acetate, glutarate, DL-3-hydroxybutyrate and 4-hydroxybutyrate | Arabidopsis thaliana | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 59000, sequence analysis | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
D-lactate cytochrome c oxidoreductase | - |
Arabidopsis thaliana |
D-LDH | - |
Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00167 | - |
glycolate | - |
Arabidopsis thaliana | |
0.1 | - |
D-glycerate | - |
Arabidopsis thaliana | |
0.1167 | - |
L-lactate | - |
Arabidopsis thaliana | |
1.083 | - |
D-lactate | - |
Arabidopsis thaliana | |
1.467 | - |
D-2-hydroxybutyrate | - |
Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | 9 | pH optimum for D-lactate, L-lactate and glycolate oxidation | Arabidopsis thaliana |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7.25 | - |
retains at least 25% activity down to pH 7.25, whereas it rapidly loses activity at lower pH values | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Arabidopsis thaliana | |
additional information | no activity with NAD+ or NADP+ as electron acceptors | Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
physiological function | participates in the methylglyoxal pathway | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0033 | - |
glycolate | - |
Arabidopsis thaliana | |
0.0167 | - |
D-glycerate | - |
Arabidopsis thaliana | |
0.033 | - |
L-lactate | - |
Arabidopsis thaliana | |
6.583 | - |
D-lactate | - |
Arabidopsis thaliana | |
24.17 | - |
D-2-hydroxybutyrate | - |
Arabidopsis thaliana |