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Literature summary for 1.1.2.4 extracted from

  • Engqvist, M.; Drincovich, M.F.; Fluegge, U.I.; Maurino, V.G.
    Two D-2-hydroxy-acid dehydrogenases in Arabidopsis thaliana with catalytic capacities to participate in the last reactions of the methylglyoxal and beta-oxidation pathways (2009), J. Biol. Chem., 284, 25026-25037.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
into vector pET16 and expressed in Escherichia coli BLR DE3 pLysS Arabidopsis thaliana

Protein Variants

Protein Variants Comment Organism
additional information mRNAs are present in both d2hgdh mutant lines, but not in dldh1-1 and dldh1-2 mutant lines Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.061
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana
0.164
-
D-lactate
-
Arabidopsis thaliana
0.432
-
glycolate
-
Arabidopsis thaliana
4.486
-
L-lactate
-
Arabidopsis thaliana
8.871
-
D-glycerate
-
Arabidopsis thaliana

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
59000
-
2 * 59000, sequence analysis Arabidopsis thaliana
135000
-
gel filtration Arabidopsis thaliana

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana Q94AX4
-
-

Purification (Commentary)

Purification (Comment) Organism
by centrifugation, sonication and on Ni2+-NTA column, to near homogeneity Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-2-hydroxybutyrate + ferricytochrome c 135% relative activity compared to (R)lactate, highest catalytic efficiency, prefered substrate Arabidopsis thaliana ?
-
?
D-2-hydroxyglutarate + ferricytochrome c 0.15% relative activity compared to (R)lactate Arabidopsis thaliana ?
-
?
D-glycerate + ferricytochrome c 9.2% relative activity compared to (R)lactate Arabidopsis thaliana ?
-
?
D-lactate + 2 ferricytochrome c 100% relative activity, prefered substrate, with a high catalytic efficiency 200- and 2000fold higher than that for L-lactate and glycolate, respectively Arabidopsis thaliana pyruvate + 2 ferrocytochrome c
-
?
DL-glycerate + ferricytochrome c 10.3% relative activity compared to (R)lactate Arabidopsis thaliana ?
-
?
glycolate + ferricytochrome c 0.15% relative activity compared to (R)lactate, O2 does not function as the acceptor for glycolate electrons Arabidopsis thaliana ?
-
?
L-lactate + ferricytochrome c 10.8% relative activity compared to (R)lactate Arabidopsis thaliana ?
-
?
additional information no activity with propionate, acetate, glutarate, DL-3-hydroxybutyrate and 4-hydroxybutyrate Arabidopsis thaliana ?
-
?

Subunits

Subunits Comment Organism
homodimer 2 * 59000, sequence analysis Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
D-lactate cytochrome c oxidoreductase
-
Arabidopsis thaliana
D-LDH
-
Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.00167
-
glycolate
-
Arabidopsis thaliana
0.1
-
D-glycerate
-
Arabidopsis thaliana
0.1167
-
L-lactate
-
Arabidopsis thaliana
1.083
-
D-lactate
-
Arabidopsis thaliana
1.467
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8 9 pH optimum for D-lactate, L-lactate and glycolate oxidation Arabidopsis thaliana

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.25
-
retains at least 25% activity down to pH 7.25, whereas it rapidly loses activity at lower pH values Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
FAD
-
Arabidopsis thaliana
additional information no activity with NAD+ or NADP+ as electron acceptors Arabidopsis thaliana

General Information

General Information Comment Organism
physiological function participates in the methylglyoxal pathway Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0033
-
glycolate
-
Arabidopsis thaliana
0.0167
-
D-glycerate
-
Arabidopsis thaliana
0.033
-
L-lactate
-
Arabidopsis thaliana
6.583
-
D-lactate
-
Arabidopsis thaliana
24.17
-
D-2-hydroxybutyrate
-
Arabidopsis thaliana