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Literature summary for 1.1.2.4 extracted from
- Electron acquisition system constructed from an NAD-independent D-lactate dehydrogenase and cytochrome c2 in Rhodopseudomonas palustris No. 7 (2004), Biosci. Biotechnol. Biochem., 68, 516-522.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| oxalate | competitive | Rhodopseudomonas palustris |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.8 | - |
D-lactate | pH 8.0, 30°C | Rhodopseudomonas palustris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 57000 | - |
4 * 57000, SDS-PAGE | Rhodopseudomonas palustris |
| 235000 | - |
gel filtration | Rhodopseudomonas palustris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Rhodopseudomonas palustris | - |
No. 7, growth photoanaerobically on lactate | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Rhodopseudomonas palustris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + ferricytochrome c | - |
Rhodopseudomonas palustris | pyruvate + ferrocytochrome c | - |
? | |
| DL-2-hydroxybutyrate + ferricytochrome c | 40% of the activity with (R)-lactate | Rhodopseudomonas palustris | 2-oxobutanoate + ferrocytochrome c | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 57000, SDS-PAGE | Rhodopseudomonas palustris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Rhodopseudomonas palustris |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
rapid inactivation above | Rhodopseudomonas palustris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Rhodopseudomonas palustris |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 9.5 | - |
Rhodopseudomonas palustris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| FAD | - |
Rhodopseudomonas palustris | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.12 | - |
oxalate | pH 8.0, 30°C | Rhodopseudomonas palustris | |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Rhodopseudomonas palustris | - |
- |
5 |
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