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Literature summary for 1.1.2.4 extracted from
- D(-)-lactic cytochrome c reductase, a flavoprotein from yeast (1961), J. Biol. Chem., 236, 920-925.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| DL-glycerate | strong inhibition | Saccharomyces cerevisiae |  |
| oxalate | competitive | Saccharomyces cerevisiae | |
| oxaloacetate | competitive | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.055 | - |
D-lactate | - |
Saccharomyces cerevisiae | |
| 1.34 | - |
D-2-hydroxybutyrate | - |
Saccharomyces cerevisiae | |
| 2.5 | - |
D-2-hydroxyvalerate | - |
Saccharomyces cerevisiae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| ferricyanide | can substitute for cytochrome c | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | - |
pyruvate + ferrocytochrome c | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 87 | - |
purified enzyme | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
? | |
| (R)-lactate + ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
? | |
| D-2-hydroxybutyrate + cytochrome c | - |
Saccharomyces cerevisiae | 2-oxobutanoate + ferrocytochrome c | - |
? | |
| D-2-hydroxyvalerate + cytochrome c | very poor substrate | Saccharomyces cerevisiae | 2-oxovalerate + ferrocytochrome c | - |
? | |
| DL-glycerate + ferricyanide | 10% of the rate of D-lactate oxidation | Saccharomyces cerevisiae | ? | - |
? | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 250 | - |
D-lactate | per mol flavin | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
with ferricyanide | Saccharomyces cerevisiae |
| 7.5 | - |
with cytochrome c | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 8.5 | low activity with cytochrome c at pH 5.5, almost maximum activity at pH 8.5 | Saccharomyces cerevisiae |
| 5.5 | 7.5 | very low activity with ferricyanide | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| 2,6-dichlorophenolindophenol | can substitute for cytochrome c | Saccharomyces cerevisiae | |
| cytochrome c | - |
Saccharomyces cerevisiae | |
| FAD | - |
Saccharomyces cerevisiae | |
Ki Value [mM]
| Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0016 | - |
oxalate | - |
Saccharomyces cerevisiae | |
| 0.01 | - |
oxaloacetate | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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