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Literature summary for 1.1.2.4 extracted from
- The lactic dehydrogenase of yeast. III. D(-)lactic cytochrome c reductase, a zinc-flavoprotein from aerobic yeast (1963), Biochim. Biophys. Acta, 67, 201-218.
General Stability
| General Stability | Organism |
|---|---|
| very stable in cold | Saccharomyces cerevisiae |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | - |
Saccharomyces cerevisiae |  |
| o-phenanthroline | very strong | Saccharomyces cerevisiae | |
| oxalate | - |
Saccharomyces cerevisiae | |
| p-Mercuriphenylsulfonate | - |
Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0054 | - |
cytochrome c | from horse heart | Saccharomyces cerevisiae | |
| 0.285 | - |
D-lactate | - |
Saccharomyces cerevisiae | |
| 1.4 | - |
D-2-hydroxybutyrate | - |
Saccharomyces cerevisiae | |
| 4.45 | - |
oxidized phenazine methosulfate | - |
Saccharomyces cerevisiae |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| mitochondrion | - |
Saccharomyces cerevisiae | 5739 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Zn2+ | very tightly bound to enzyme | Saccharomyces cerevisiae | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + 2 ferricytochrome c | Saccharomyces cerevisiae | - |
pyruvate + ferrocytochrome c | - |
ir | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1670 | - |
purified enzyme | Saccharomyces cerevisiae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, as ammonium sulfate precipitate | Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (R)-lactate + 2 ferricytochrome c | - |
Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
ir | |
| (R)-lactate + ferricytochrome c | specific for D-isomer | Saccharomyces cerevisiae | pyruvate + ferrocytochrome c | - |
ir | |
| D-2-hydroxybutyrate + cytochrome c | - |
Saccharomyces cerevisiae | 2-oxobutanoate + ferrocytochrome c | - |
? | |
| D-lactate + oxidized phenazine methosulfate | - |
Saccharomyces cerevisiae | pyruvate + reduced phenazine methosulfate + H+ | - |
r | |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1500 | - |
D-lactate | per mol flavin | Saccharomyces cerevisiae | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
phosphate buffer | Saccharomyces cerevisiae |
| 7.5 | - |
imidazole and Tris buffer | Saccharomyces cerevisiae |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | - |
stable for short term exposure (30 s) | Saccharomyces cerevisiae |
| 6.5 | - |
very stable | Saccharomyces cerevisiae |
| 7.5 | - |
complete inactivation above pH 7.5 | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| cytochrome c | cytochome c from horse-, beef- and tuna-heart more active than yeast cytochome c | Saccharomyces cerevisiae | |
| FAD | - |
Saccharomyces cerevisiae | |
| phenazine methosulfate | can substitute for cytochrome c | Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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