Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.2.3 extracted from

  • Smutok, O.; Karkovska, M.; Smutok, H.; Gonchar, M.
    Flavocytochrome b2-based enzymatic method of L-lactate assay in food products (2013), ScientificWorldJournal, 2013, 461284 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
analysis enzymatic oxidation of L-lactate catalyzed by flavocytochrome b2 and coupled with formazan production from nitrotetrazolium blue can be used for L-lactate assay in food samples. A high correlation between results of the proposed method and reference ones proves the possibility to use flavocytochrome b2-catalysed reaction for enzymatic measurement of L-lactate in biotechnology and food chemistry Ogataea angusta

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
-
Ogataea angusta 5739
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Ogataea angusta
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c Ogataea angusta tr1
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 flavocytochrome b2 Ogataea angusta
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 flavocytochrome b2 Ogataea angusta tr1
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Ogataea angusta W1QKE8
-
-
Ogataea angusta tr1 W1QKE8
-
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme by anion exchange chromatography Ogataea angusta

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
11
-
crude enzyme, cell-free extracts, pH 7.8, 20°C Ogataea angusta

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
Ogataea angusta pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c
-
Ogataea angusta tr1 pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 flavocytochrome b2
-
Ogataea angusta pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 flavocytochrome b2
-
Ogataea angusta tr1 pyruvate + 2 ferrocytochrome c + 2 H+
-
?
additional information development and evaluation of an enzymatic method exploiting an enzymatic oxidation of L-lactate to pyruvate coupled with nitrotetrazolium blue (NTZB) reduction to a colored product, formazan, overview. The main advantages of the proposed method when compared to the LDH (EC 1.1.1.27)-based routine approaches are a higher sensitivity, simple procedure of analysis, usage of inexpensive, nontoxic reagents, and small amount of the enzyme. The enzyme is absolutely specific for L-lactate Ogataea angusta ?
-
?
additional information development and evaluation of an enzymatic method exploiting an enzymatic oxidation of L-lactate to pyruvate coupled with nitrotetrazolium blue (NTZB) reduction to a colored product, formazan, overview. The main advantages of the proposed method when compared to the LDH (EC 1.1.1.27)-based routine approaches are a higher sensitivity, simple procedure of analysis, usage of inexpensive, nontoxic reagents, and small amount of the enzyme. The enzyme is absolutely specific for L-lactate Ogataea angusta tr1 ?
-
?

Subunits

Subunits Comment Organism
homotetramer the enzyme is a tetramer with four identical subunits, each consisting of FMN- and heme-binding domains Ogataea angusta

Synonyms

Synonyms Comment Organism
FC b2
-
Ogataea angusta
flavocytochrome b2
-
Ogataea angusta
L-lactate-cytochrome c oxidoreductase
-
Ogataea angusta

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
20
-
assay at Ogataea angusta

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
assay at Ogataea angusta

Cofactor

Cofactor Comment Organism Structure
ferricytochrome c
-
Ogataea angusta
flavocytochrome b2
-
Ogataea angusta
FMN each of the four subunits contains a flavin mononucleotide prosthetic group Ogataea angusta
heme each of the four subunits contains a beta-type heme Ogataea angusta

General Information

General Information Comment Organism
physiological function L-lactate-cytochrome b oxidoreductase catalyses the electron transfer from L-lactate to cytochrome b in yeast mitochondria Ogataea angusta