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Literature summary for 1.1.2.3 extracted from

  • Sharp, R.E.; Chapman, S.K.; Reid, G.A.
    Deletions in the interdomain hinge region of flavocytochrome b2: Effects on intraprotein electron transfer (1996), Biochemistry, 35, 891-899.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
in Escherichia coli AR120 Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
additional information three different deletion mutants with deletion of 3, 6 and 9 amino acids from hinge region Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
4 * 57000 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae ?
-
?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 57000 Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
additional information systematic determination of turnover numbers for wild type enzyme and deletion mutants with different electron acceptors, significantly reduced activity for deletion mutants with cytochrome c as electron acceptor Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
cytochrome c
-
Saccharomyces cerevisiae
additional information ferricyanide used as electron acceptor Saccharomyces cerevisiae
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae