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Literature summary for 1.1.2.3 extracted from

  • Labeyrie, F.; Baudras, A.; Lederer, F.
    Flavocytochrome b 2 or L-lactate cytochrome c reductase from yeast (1978), Methods Enzymol., 53, 238-256.
    View publication on PubMed

Crystallization (Commentary)

Crystallization (Comment) Organism
used as essential purification step, crystallized as DNA complex Saccharomyces cerevisiae

General Stability

General Stability Organism
intact enzyme unstable at low ionic strength Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
D-lactate
-
Saccharomyces cerevisiae
D-lactate
-
Wickerhamomyces anomalus
L-lactate 60% inhibition at 10 mM, no inhibition with cleaved form of enzyme Saccharomyces cerevisiae
L-lactate
-
Wickerhamomyces anomalus
oxalate
-
Saccharomyces cerevisiae
oxalate
-
Wickerhamomyces anomalus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
L-lactate
-
Wickerhamomyces anomalus
0.4
-
L-lactate intact enzyme Saccharomyces cerevisiae
1.3
-
L-lactate
-
Wickerhamomyces anomalus
1.6
-
L-lactate cleaved enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ bound to enzyme Saccharomyces cerevisiae
Fe2+ bound to enzyme Wickerhamomyces anomalus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
57000
-
4 * 57000 Saccharomyces cerevisiae
60000
-
4 * 60000 Wickerhamomyces anomalus
220000
-
-
Wickerhamomyces anomalus
220000
-
intact enzyme Saccharomyces cerevisiae
230000
-
cleaved enzyme Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-lactate + 2 ferricytochrome c Wickerhamomyces anomalus
-
pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c Saccharomyces cerevisiae can feed electrons to respiratory chain at the level of cytochrome c pyruvate + 2 ferrocytochrome c + 2 H+
-
?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Wickerhamomyces anomalus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Wickerhamomyces anomalus
purification in presence and absence of PMSF results intact or cleaved enzyme Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
20°C, 0.2 M phosphate buffer, pH 7.2, 1 mM EDTA, protected from light, 50% loss of activity within 10-20 days Wickerhamomyces anomalus
under nitrogen, 1 month, no loss of activity Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-lactate + 2 ferricytochrome c
-
Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c
-
Wickerhamomyces anomalus pyruvate + 2 ferrocytochrome c + 2 H+
-
?
(S)-lactate + 2 ferricytochrome c can feed electrons to respiratory chain at the level of cytochrome c Saccharomyces cerevisiae pyruvate + 2 ferrocytochrome c + 2 H+
-
?
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae ?
-
?
additional information electron acceptors other than ferricytochrome c used Wickerhamomyces anomalus ?
-
?

Subunits

Subunits Comment Organism
tetramer 4 * 57000 Saccharomyces cerevisiae
tetramer 4 * 60000 Wickerhamomyces anomalus
tetramer 4 * 34000-36000 + 4 * 21000-22000, cleaved enzyme Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
cleaved enzyme Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
FMN one FMN per subunit Saccharomyces cerevisiae
FMN one FMN per subunit Wickerhamomyces anomalus
heme one heme per subunit Saccharomyces cerevisiae
heme one heme per subunit Wickerhamomyces anomalus
additional information electron acceptors other than ferricytochrome c used Saccharomyces cerevisiae
additional information electron acceptors other than ferricytochrome c used Wickerhamomyces anomalus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.5
-
oxalate intact enzyme Saccharomyces cerevisiae
0.7
-
oxalate
-
Wickerhamomyces anomalus
1.3
-
oxalate cleaved enzyme Saccharomyces cerevisiae
1.4
-
D-lactate intact enzyme Saccharomyces cerevisiae
5.3
-
D-lactate
-
Wickerhamomyces anomalus
6
-
D-lactate cleaved enzyme Saccharomyces cerevisiae
45
-
L-lactate
-
Wickerhamomyces anomalus