Literature summary for 1.1.2.10 extracted from

Good, N.M.; Moore, R.S.; Suriano, C.J.; Martinez-Gomez, N.C.
Contrasting in vitro and in vivo methanol oxidation activities of lanthanide-dependent alcohol dehydrogenases XoxF1 and ExaF from Methylobacterium extorquens AM1 (2019), Sci. Rep., 9, 4248 .

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KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.011	-	methanol	XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication	Methylorubrum extorquens
0.014	-	ethanol	XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication	Methylorubrum extorquens
0.029	-	methanol	XoxF1, in the presence of Nd3+, pH and temperature not specified in the publication	Methylorubrum extorquens
0.044	-	methanol	XoxF1, in the presence of La3+, pH and temperature not specified in the publication	Methylorubrum extorquens
0.065	-	formaldehyde	XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication	Methylorubrum extorquens
0.067	-	ethanol	XoxF1, in the presence of La3+, pH and temperature not specified in the publication	Methylorubrum extorquens
0.096	-	formaldehyde	XoxF1, in the presence of La3+, pH and temperature not specified in the publication	Methylorubrum extorquens
0.133	-	formaldehyde	XoxF1, in the presence of Nd3+, pH and temperature not specified in the publication	Methylorubrum extorquens

Metals/Ions

Metals/Ions	Comment	Organism	Structure
La3+	dependent on. XoxF1 contains La3+ in 1:1 molar ratio of metal to protomer	Methylorubrum extorquens

Organism

Organism	UniProt	Comment	Textmining
Methylorubrum extorquens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ethanol + phenazine methosulfate	best substrate for ExaF	Methylorubrum extorquens	acetaldehyde + reduced phenazine methosulfate	-	?
methanol + phenazine methosulfate	best substrate for XoxF1	Methylorubrum extorquens	formaldehyde + reduced phenazine methosulfate	-	?

Synonyms

Synonyms	Comment	Organism
exaF	-	Methylorubrum extorquens
lanthanide-dependent alcohol dehydrogenase	-	Methylorubrum extorquens
Ln-dependent EtDH	-	Methylorubrum extorquens
Ln-dependent ethanol dehydrogenase	-	Methylorubrum extorquens
Ln-dependent MetDH	-	Methylorubrum extorquens
Ln-dependent methanol dehydrogenase	-	Methylorubrum extorquens
xoxF1	-	Methylorubrum extorquens

Cofactor

Cofactor	Comment	Organism	Structure
pyrroloquinoline quinone	-	Methylorubrum extorquens

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
3	-	methanol	XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication	Methylorubrum extorquens
4	-	ethanol	XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication	Methylorubrum extorquens
5	-	formaldehyde	XoxF1, in the absence of lanthanides, pH and temperature not specified in the publication	Methylorubrum extorquens
36	-	formaldehyde	XoxF1, in the presence of Nd3+, pH and temperature not specified in the publication	Methylorubrum extorquens
109	-	formaldehyde	XoxF1, in the presence of La3+, pH and temperature not specified in the publication	Methylorubrum extorquens
194	-	ethanol	XoxF1, in the presence of La3+, pH and temperature not specified in the publication	Methylorubrum extorquens
210	-	methanol	XoxF1, in the presence of Nd3+, pH and temperature not specified in the publication	Methylorubrum extorquens
272	-	methanol	XoxF1, in the presence of La3+, pH and temperature not specified in the publication	Methylorubrum extorquens

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Release 2023.1 (January 2023)