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Literature summary for 1.1.1.B57 extracted from

  • Vitale, A.; Rosso, F.; Barbarisi, A.; Labella, T.; D'Auria, S.
    Properties and evolution of an alcohol dehydrogenase from the Crenarchaeota Pyrobaculum aerophilum (2010), Gene, 461, 26-31.
    View publication on PubMed
Search for more literature for 1.1.1.B57

Cloned(Commentary)

Cloned (Comment) Organism
overexpression in Escherichia coli Pyrobaculum aerophilum

General Stability

General Stability Organism
Zn2+ is not essential for structural stability of the enzyme Pyrobaculum aerophilum

Inhibitors

Inhibitors Comment Organism Structure
EDTA 1 mM, 20% decrease in activity Pyrobaculum aerophilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4
-
 3,4-dihydro-1(2H)-naphthalenone 95°C, pH 7.5 Pyrobaculum aerophilum

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ important for activity Pyrobaculum aerophilum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38580
-
 x * 38580, calculated from sequence Pyrobaculum aerophilum
40000
-
 x * 40000, SDS-PAGE Pyrobaculum aerophilum

Organism

Organism UniProt Comment Textmining
Pyrobaculum aerophilum Q8ZUP0
-
-
Pyrobaculum aerophilum DSM 7523 Q8ZUP0
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Pyrobaculum aerophilum

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
 60°C, pH 7.5 Pyrobaculum aerophilum
50
-
 95°C, pH 7.5 Pyrobaculum aerophilum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+ 3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate Pyrobaculum aerophilum 1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
 ?
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+ 3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate Pyrobaculum aerophilum DSM 7523 1,2,3,4-tetrahydronaphthalen-1-ol + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 38580, calculated from sequence Pyrobaculum aerophilum
? x * 40000, SDS-PAGE Pyrobaculum aerophilum

Synonyms

Synonyms Comment Organism
PAE2687 locus name Pyrobaculum aerophilum
PyAeADHII
-
 Pyrobaculum aerophilum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
 reaction rate increases with temperature up to 95°C Pyrobaculum aerophilum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1719
-
 3,4-dihydro-1(2H)-naphthalenone 95°C, pH 7.5 Pyrobaculum aerophilum