Application | Comment | Organism |
---|---|---|
pharmacology | stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals | Rhodotorula mucilaginosa |
synthesis | stereospecific production of (R)-3-quinuclidinol, an important chiral building block for the synthesis of various pharmaceuticals | Rhodotorula mucilaginosa |
Cloned (Comment) | Organism |
---|---|
an expression vector, pWKLQ, which contains the full length 3-quinuclidinone reductase gene is constructed. Using Escherichia coli cells coexpressing the 3-quinuclidinone reductase and glucose dehydrogenase (cofactor regeneration enzyme) genes, 618 mM 3-quinuclidinone is almost stiochiometrically converted to (R)-3-quinuclidinol with an >99.9% enantiomeric excess within 21 h of reaction | Rhodotorula mucilaginosa |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-mercuribenzoate | 1 mM, complete inhibition | Rhodotorula mucilaginosa | |
5,5'-dithiobis(2-nitrobenzoate) | 1 mM, 15% inhibition | Rhodotorula mucilaginosa | |
Hg2+ | 1 mM, complete inhibition | Rhodotorula mucilaginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
NADPH | pH 7.0, temperature not specified in the publication | Rhodotorula mucilaginosa | |
145 | - |
3-quinuclidinone | pH 7.0, temperature not specified in the publication | Rhodotorula mucilaginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
30000 | - |
3 or 4 * 3 * 30000, SDS-PAGE | Rhodotorula mucilaginosa |
93000 | - |
gel filtration | Rhodotorula mucilaginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula mucilaginosa | B9ZZZ6 | - |
- |
Rhodotorula mucilaginosa JCM 3782 | B9ZZZ6 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhodotorula mucilaginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
341 | - |
pH 7.0, temperature not specified in the publication | Rhodotorula mucilaginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-quinuclidinone + NADPH + H+ | - |
Rhodotorula mucilaginosa | (R)-3-quinuclidinol + NADP+ | - |
? | |
3-quinuclidinone + NADPH + H+ | - |
Rhodotorula mucilaginosa JCM 3782 | (R)-3-quinuclidinol + NADP+ | - |
? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Rhodotorula mucilaginosa | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
? | |
ethyl 4-chloro-3-oxobutanoate + NADPH + H+ | - |
Rhodotorula mucilaginosa JCM 3782 | ethyl (S)-4-chloro-3-hydroxybutanoate + NADP+ | - |
? | |
ketopantoyl lactone + NADPH + H+ | the optical purity of pantoyl lactone is only 17.2% ee for L-isomer | Rhodotorula mucilaginosa | pantoyl lactone + NADP+ | - |
? | |
ketopantoyl lactone + NADPH + H+ | the optical purity of pantoyl lactone is only 17.2% ee for L-isomer | Rhodotorula mucilaginosa JCM 3782 | pantoyl lactone + NADP+ | - |
? | |
additional information | no activity with: acetaldehyde, propionaldehyde, heptanal, glyoxal, benzaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, 2-chlorobenzaldehyde, 3-chlorobenzaldehyde, pyridine-3-aldehyde, 2,4-pentanedione, 3-methylcyclohexanone, 4-methylcyclohexanone, 4-ethylcyclohexanone, isatin, p-toluquinone, alpha-naphthoquinone, menadione, ketoisophorone, levodione, 4-piperidone hydrochloride monohydrate, 1-methyl-4-piperidone, and tropinone | Rhodotorula mucilaginosa | ? | - |
? | |
additional information | no activity with: acetaldehyde, propionaldehyde, heptanal, glyoxal, benzaldehyde, 2-nitrobenzaldehyde, 3-nitrobenzaldehyde, 4-nitrobenzaldehyde, 2-chlorobenzaldehyde, 3-chlorobenzaldehyde, pyridine-3-aldehyde, 2,4-pentanedione, 3-methylcyclohexanone, 4-methylcyclohexanone, 4-ethylcyclohexanone, isatin, p-toluquinone, alpha-naphthoquinone, menadione, ketoisophorone, levodione, 4-piperidone hydrochloride monohydrate, 1-methyl-4-piperidone, and tropinone | Rhodotorula mucilaginosa JCM 3782 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | 3 or 4 * 3 * 30000, SDS-PAGE | Rhodotorula mucilaginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Rhodotorula mucilaginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Rhodotorula mucilaginosa |
7 | - |
assay at | Rhodotorula mucilaginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | highly specific for NADPH as a coenzyme | Rhodotorula mucilaginosa |