Crystallization (Comment) | Organism |
---|---|
sitting-drop vapor-diffusion method with a reservoir solution containing 100 mM CHES (pH 10.0), 30% PEG8000, and 3% sucrose, at 5°C | Rhodotorula mucilaginosa |
Protein Variants | Comment | Organism |
---|---|---|
F212A | complete loss of activity | Rhodotorula mucilaginosa |
F212L | complete loss of activity | Rhodotorula mucilaginosa |
I167A | complete loss of activity | Rhodotorula mucilaginosa |
I167V | relative activity for 3-qinuclidinone is 24.5% compared to wild-type enzyme | Rhodotorula mucilaginosa |
K185A | complete loss of activity | Rhodotorula mucilaginosa |
N173A | relative activity for 3-qinuclidinone is 97.8% compared to wild-type enzyme | Rhodotorula mucilaginosa |
Q178A | relative activity for 3-qinuclidinone is 164.5% compared to wild-type enzyme | Rhodotorula mucilaginosa |
S166A | complete loss of activity | Rhodotorula mucilaginosa |
S168A | relative activity for 3-qinuclidinone is 115.7% compared to wild-type enzyme | Rhodotorula mucilaginosa |
Y181A | complete loss of activity | Rhodotorula mucilaginosa |
Y181F | complete loss of activity | Rhodotorula mucilaginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
3-quinuclidinone | pH 7.0, 30°C, mutant enzyme N173A | Rhodotorula mucilaginosa | |
0.103 | - |
3-quinuclidinone | pH 7.0, 30°C, mutant enzyme Q178A | Rhodotorula mucilaginosa | |
0.168 | - |
3-quinuclidinone | pH 7.0, 30°C, mutant enzyme S168A | Rhodotorula mucilaginosa | |
0.308 | - |
3-quinuclidinone | pH 7.0, 30°C, mutant enzyme I167V | Rhodotorula mucilaginosa | |
0.44 | - |
3-quinuclidinone | pH 7.0, 30°C, wild-type enzyme | Rhodotorula mucilaginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodotorula mucilaginosa | B9ZZZ6 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-quinuclidinone + NADPH + H+ | - |
Rhodotorula mucilaginosa | (R)-3-quinuclidinol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | - |
Rhodotorula mucilaginosa |
Synonyms | Comment | Organism |
---|---|---|
RrQR | - |
Rhodotorula mucilaginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhodotorula mucilaginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.69 | - |
3-quinuclidinone | pH 7.0, 30°C, mutant enzyme I167V | Rhodotorula mucilaginosa | |
5.62 | - |
3-quinuclidinone | pH 7.0, 30°C, mutant enzyme N173A | Rhodotorula mucilaginosa | |
9.64 | - |
3-quinuclidinone | pH 7.0, 30°C, mutant enzyme S168A | Rhodotorula mucilaginosa | |
9.79 | - |
3-quinuclidinone | pH 7.0, 30°C, mutant enzyme Q178A | Rhodotorula mucilaginosa | |
15.1 | - |
3-quinuclidinone | pH 7.0, 30°C, wild-type enzyme | Rhodotorula mucilaginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Rhodotorula mucilaginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | residues R60 and S61 form hydrogen bonds with the 2'-phosphate group of the NADPH. These bonds are involved in the discrimination of NADPH from NADH | Rhodotorula mucilaginosa |