Application | Comment | Organism |
---|---|---|
synthesis | Bacillus licheniformis strain MW3 (DELTAbudCDELTAgdh) can be useful for the production of acetoin on a commercial scale | Bacillus licheniformis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a knockout Bacillus licheniformis mutant DELTAbudCDELTAgdh deleting two 2,3-butanediol dehydrogenases, i.e. meso-2,3-butanediol dehydrogenases BudC and GDH, through gene disruption. Escherichia coli strain S17-1 lpir is used as donor strain to allow the conjugal transfer of plasmids pKVM1-1budC and pKVM1-1gdh into Bacillus licheniformis strain MW3. Although the growth of strain MW3 (DELTAbudCDELTAgdh) is slightly lower than that of wild-type strain MW3, it can produce acetoin instead of 2,3-butanediol as its major product. Using fedbatch fermentation of Bacillus licheniformis MW3 (DELTAbudCDELTAgdh), 64.2 g/l acetoin is produced at a productivity of 2.378 g/l/h and a yield of 0.412 g/g from 156 g/l glucose in 27 h | Bacillus licheniformis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-acetoin + NADH + H+ | Bacillus licheniformis | - |
(2R,3S)-butane-2,3-diol + NAD+ | - |
r | |
(3R)-acetoin + NADH + H+ | Bacillus licheniformis MW3 | - |
(2R,3S)-butane-2,3-diol + NAD+ | - |
r | |
additional information | Bacillus licheniformis | most of the Bacillus strains produce 2,3-butanediol but not acetoin as their major product | ? | - |
- |
|
additional information | Bacillus licheniformis MW3 | most of the Bacillus strains produce 2,3-butanediol but not acetoin as their major product | ? | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | - |
- |
- |
Bacillus licheniformis MW3 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | batch and fed-batch fermentations in bioreactors of 1-5 l volume | Bacillus licheniformis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(3R)-acetoin + NADH + H+ | - |
Bacillus licheniformis | (2R,3S)-butane-2,3-diol + NAD+ | - |
r | |
(3R)-acetoin + NADH + H+ | - |
Bacillus licheniformis MW3 | (2R,3S)-butane-2,3-diol + NAD+ | - |
r | |
additional information | most of the Bacillus strains produce 2,3-butanediol but not acetoin as their major product | Bacillus licheniformis | ? | - |
- |
|
additional information | most of the Bacillus strains produce 2,3-butanediol but not acetoin as their major product | Bacillus licheniformis MW3 | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
2,3-butanediol dehydrogenase | - |
Bacillus licheniformis |
budC | - |
Bacillus licheniformis |
More | cf. EC 1.1.1.4 and EC 1.1.1.76 | Bacillus licheniformis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bacillus licheniformis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Bacillus licheniformis | |
NADH | - |
Bacillus licheniformis |
General Information | Comment | Organism |
---|---|---|
malfunction | the growth of Bacillus licheniformis mutant strain MW3 (DELTAbudCDELTAgdh) is slightly lower than that of Bacillus licheniformis wild-type strain MW3, but the mutant strain can produce acetoin instead of 2,3-butanediol as its major product | Bacillus licheniformis |