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Literature summary for 1.1.1.B20 extracted from

  • Lue, C.; Ge, Y.; Cao, M.; Guo, X.; Liu, P.; Gao, C.; Xu, P.; Ma, C.
    Metabolic engineering of Bacillus licheniformis for production of acetoin (2020), Front. Bioeng. Biotechnol., 8, 125 .
    View publication on PubMedView publication on EuropePMC

Application

Application Comment Organism
synthesis Bacillus licheniformis strain MW3 (DELTAbudCDELTAgdh) can be useful for the production of acetoin on a commercial scale Bacillus licheniformis

Protein Variants

Protein Variants Comment Organism
additional information construction of a knockout Bacillus licheniformis mutant DELTAbudCDELTAgdh deleting two 2,3-butanediol dehydrogenases, i.e. meso-2,3-butanediol dehydrogenases BudC and GDH, through gene disruption. Escherichia coli strain S17-1 lpir is used as donor strain to allow the conjugal transfer of plasmids pKVM1-1budC and pKVM1-1gdh into Bacillus licheniformis strain MW3. Although the growth of strain MW3 (DELTAbudCDELTAgdh) is slightly lower than that of wild-type strain MW3, it can produce acetoin instead of 2,3-butanediol as its major product. Using fedbatch fermentation of Bacillus licheniformis MW3 (DELTAbudCDELTAgdh), 64.2 g/l acetoin is produced at a productivity of 2.378 g/l/h and a yield of 0.412 g/g from 156 g/l glucose in 27 h Bacillus licheniformis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(3R)-acetoin + NADH + H+ Bacillus licheniformis
-
(2R,3S)-butane-2,3-diol + NAD+
-
r
(3R)-acetoin + NADH + H+ Bacillus licheniformis MW3
-
(2R,3S)-butane-2,3-diol + NAD+
-
r
additional information Bacillus licheniformis most of the Bacillus strains produce 2,3-butanediol but not acetoin as their major product ?
-
-
additional information Bacillus licheniformis MW3 most of the Bacillus strains produce 2,3-butanediol but not acetoin as their major product ?
-
-

Organism

Organism UniProt Comment Textmining
Bacillus licheniformis
-
-
-
Bacillus licheniformis MW3
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information batch and fed-batch fermentations in bioreactors of 1-5 l volume Bacillus licheniformis
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(3R)-acetoin + NADH + H+
-
Bacillus licheniformis (2R,3S)-butane-2,3-diol + NAD+
-
r
(3R)-acetoin + NADH + H+
-
Bacillus licheniformis MW3 (2R,3S)-butane-2,3-diol + NAD+
-
r
additional information most of the Bacillus strains produce 2,3-butanediol but not acetoin as their major product Bacillus licheniformis ?
-
-
additional information most of the Bacillus strains produce 2,3-butanediol but not acetoin as their major product Bacillus licheniformis MW3 ?
-
-

Synonyms

Synonyms Comment Organism
2,3-butanediol dehydrogenase
-
Bacillus licheniformis
budC
-
Bacillus licheniformis
More cf. EC 1.1.1.4 and EC 1.1.1.76 Bacillus licheniformis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Bacillus licheniformis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Bacillus licheniformis

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus licheniformis
NADH
-
Bacillus licheniformis

General Information

General Information Comment Organism
malfunction the growth of Bacillus licheniformis mutant strain MW3 (DELTAbudCDELTAgdh) is slightly lower than that of Bacillus licheniformis wild-type strain MW3, but the mutant strain can produce acetoin instead of 2,3-butanediol as its major product Bacillus licheniformis