Cloned (Comment) | Organism |
---|---|
gene budC , recombinant expression in Escherichia coli strain BL21(DE3) | Klebsiella aerogenes |
gene budC, recombinant expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
D194G | site-directed mutagenesis, the mutant binds the substrate but is catalytically almost inactive. The mutant is inactive with (2S,3S)-butanediol, meso-butanediol and (2R,3R)-butanediol. D194G enzyme mutant shows a similar secondary structure compared to Enterobacter aerogenes BDH. While the mutant is highly susceptible to protease digestion compared to the wild-type enzyme. Homology modeling of the mutant enzyme, with meso-2,3-butanediol dehydrogenase from Klebsiella pneumoniae, PDB ID 1GEG, as a template, reveals that Gly194 seems to lose the hydrogen bond interactions with the surrounding residues (Gly206, Gly207 and Thr209), resulting in a putative conformational changes of mutant D194G which might be responsible for the loss of activity | Bacillus subtilis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamics | Bacillus subtilis | |
additional information | - |
additional information | thermodynamics | Klebsiella aerogenes |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-acetoin + NADH + H+ | Bacillus subtilis | - |
meso-2,3-butanediol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | Klebsiella aerogenes | - |
meso-2,3-butanediol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | Klebsiella aerogenes KCTC 2190 | - |
meso-2,3-butanediol + NAD+ | - |
r | |
meso-2,3-butanediol + NAD+ | Bacillus subtilis | - |
(R)-acetoin + NADH + H+ | - |
r | |
meso-2,3-butanediol + NAD+ | Klebsiella aerogenes | - |
(R)-acetoin + NADH + H+ | - |
r | |
meso-2,3-butanediol + NAD+ | Klebsiella aerogenes KCTC 2190 | - |
(R)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Klebsiella aerogenes | A0A0H3FZT7 | - |
- |
Klebsiella aerogenes KCTC 2190 | A0A0H3FZT7 | - |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.37 | - |
purified recombinant enzyme, pH 7.6, 37°C, substrates meso-2,3-butanediol and NADP+ | Klebsiella aerogenes |
2.9 | - |
purified recombinant enzyme, pH 7.6, 37°C, substrates acetoin and NADPH | Klebsiella aerogenes |
119.55 | - |
purified recombinant enzyme, pH 7.6, 37°C, substrates meso-2,3-butanediol and NAD+ | Klebsiella aerogenes |
461.67 | - |
purified recombinant enzyme, pH 7.6, 37°C, substrates acetoin and NADH | Klebsiella aerogenes |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-acetoin + NADH + H+ | - |
Bacillus subtilis | meso-2,3-butanediol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | - |
Klebsiella aerogenes | meso-2,3-butanediol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | preferred reaction direction | Klebsiella aerogenes | meso-2,3-butanediol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | - |
Klebsiella aerogenes KCTC 2190 | meso-2,3-butanediol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | preferred reaction direction | Klebsiella aerogenes KCTC 2190 | meso-2,3-butanediol + NAD+ | - |
r | |
(R)-acetoin + NADPH + H+ | very low activity | Klebsiella aerogenes | meso-2,3-butanediol + NADP+ | - |
r | |
(R)-acetoin + NADPH + H+ | very low activity | Klebsiella aerogenes KCTC 2190 | meso-2,3-butanediol + NADP+ | - |
r | |
meso-2,3-butanediol + NAD+ | - |
Bacillus subtilis | (R)-acetoin + NADH + H+ | - |
r | |
meso-2,3-butanediol + NAD+ | - |
Klebsiella aerogenes | (R)-acetoin + NADH + H+ | - |
r | |
meso-2,3-butanediol + NAD+ | - |
Klebsiella aerogenes KCTC 2190 | (R)-acetoin + NADH + H+ | - |
r | |
meso-2,3-butanediol + NADP+ | very low activity | Klebsiella aerogenes | (R)-acetoin + NADPH + H+ | - |
r | |
additional information | meso-2,3-butanediol dehydrogenase (BDH) catalyzes the redox reaction between (R)-acetoin and meso-2,3-butanediol | Bacillus subtilis | ? | - |
? | |
additional information | the enzyme is also active with diacetyl and NADH | Klebsiella aerogenes | ? | - |
? | |
additional information | the enzyme is also active with diacetyl and NADH | Klebsiella aerogenes KCTC 2190 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
BDH | - |
Bacillus subtilis |
BDH | - |
Klebsiella aerogenes |
budC | - |
Bacillus subtilis |
budC | - |
Klebsiella aerogenes |
meso-2,3-BDH | - |
Bacillus subtilis |
meso-2,3-BDH | - |
Klebsiella aerogenes |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Bacillus subtilis |
37 | - |
assay at | Klebsiella aerogenes |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Bacillus subtilis |
7.6 | - |
assay at | Klebsiella aerogenes |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme prefers NAD+as a coenzyme | Klebsiella aerogenes | |
NAD+ | - |
Bacillus subtilis | |
NAD+ | - |
Klebsiella aerogenes | |
NADH | - |
Bacillus subtilis | |
NADH | - |
Klebsiella aerogenes | |
NADP+ | very low activity | Klebsiella aerogenes | |
NADPH | very low activity | Klebsiella aerogenes |