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Literature summary for 1.1.1.95 extracted from

  • Burton, R.L.; Chen, S.; Xu, X.L.; Grant, G.A.
    Transient kinetic analysis of the interaction of L-serine with Escherichia coli D-3-phosphoglycerate dehydrogenase reveals the mechanism of V-type regulation and the order of effector binding (2009), Biochemistry, 48, 12242-12251.
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
W139F/E360W exhibits catalytic activity comparable to that of the native enzyme and is effectively inhibited by L-serine. The only fluorescence signal of the mutant is due to the single tryptophan at position 360. Pre-steady state analysis of binding of inhibitor serine shows that each serine binding interface produces an integrated fluorescent signal Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
L-serine physiological inhibitor, exerts its effect on at least two steps in the kinetic mechanism. There is a small but significant effect on the dissociation constant of NADH, increasing the Kd to 5 and 23 microM from 0.6 and 9 microM, respectively, for the two sets of sites in the enzyme. After the second substrate is added, serine reduces the amplitude of the signal without a significant effect on the observed rate constants for binding. The serine concentration that reduces the amplitude by 50% is equal to the K0.5 for serine inhibition. Serine binding eliminates a conformational change subsequent to substrate binding by formation of a dead-end quaternary complex consisting of enzyme, coenzyme, substrate, and effector. The rate data conform to a model in which serine can bind to two forms of the enzyme with different affinities Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli P0A9T0
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxoglutarate + NADH + H+
-
Escherichia coli 2-hydroxyglutarate + NAD+
-
?

Cofactor

Cofactor Comment Organism Structure
NADH
-
Escherichia coli