Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli strain AD494 (DE3), cloning of GFP-mutant with transit peptide, expression in plant | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
additional information | expression of GFP-mutant enzyme in Arabidopsis to investigate the tissue distribution, complementation of Escherichia coli serA- mutant | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Ser, Tyr, Val, Gly, Trp, O-acetyl-L-Ser, and Cys have no effect on enzyme activity in both directions | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
NAD+ | pH 9.0 | Arabidopsis thaliana | |
0.12 | - |
NADH | pH 7.1 | Arabidopsis thaliana | |
0.35 | - |
3-phosphohydroxypyruvate | pH 7.1 | Arabidopsis thaliana | |
1.19 | - |
3-phospho-D-glycerate | pH 9.0 | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | in leaves | Arabidopsis thaliana | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66453 | - |
x * 66453 and 90000, DNA sequence analysis and SDS-PAGE | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phosphoglycerate + NAD+ | Arabidopsis thaliana | first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate | 3-phosphohydroxypyruvate + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | light-grown more than dark-grown | Arabidopsis thaliana | - |
root | light-grown more than dark-grown | Arabidopsis thaliana | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.14 | - |
3-phosphoglycerate oxidation, recombinant protein | Arabidopsis thaliana |
10.95 | - |
3-phosphohydroxypyruvate reduction, recombinant protein | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | - |
Arabidopsis thaliana | 3-phosphooxypyruvate + NADH + H+ | - |
r | |
3-phosphoglycerate + NAD+ | - |
Arabidopsis thaliana | 3-phosphohydroxypyruvate + NADH | - |
r | |
3-phosphoglycerate + NAD+ | first enzyme in metabolic sequence of synthesis of serine from 3-phosphoglycerate | Arabidopsis thaliana | 3-phosphohydroxypyruvate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 66453 and 90000, DNA sequence analysis and SDS-PAGE | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana |