Crystallization (Comment) | Organism |
---|---|
- |
Escherichia coli |
General Stability | Organism |
---|---|
dithiothreitol increases stability | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | inhibition can be overcome by addition of dithiothreitol | Escherichia coli | |
L-serine | inhibition of enzyme from E. coli, Salmonella typhimurium and Haemophilus influenzae, not of mammalian enzyme, inhibition in both reaction directions | Escherichia coli | |
Mercurials | inhibition can be overcome by addition of dithiothreitol | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0013 | - |
3-phosphohydroxypyruvate | Km at pH 7.5 is lower than at pH 8.8 | Escherichia coli | |
0.0078 | - |
NAD+ | Km at pH 7.5 is lower than at pH 8.8 | Escherichia coli | |
1.1 | - |
D-3-phosphoglycerate | - |
Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
163000 | - |
sedimentation equilibrium analysis | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-phospho-D-glycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
? | |
3-phosphoglycerate + NAD+ | - |
Escherichia coli | 3-phosphohydroxypyruvate + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000-50000, sedimentation equilibrium in guanidine-HCl | Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
initial rate maximum, but enzyme not stable | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
3-acetylpyridine-NADH | 40% as effective as NADH | Escherichia coli | |
deamino-NADH | as effective as NADH | Escherichia coli | |
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli | |
NADPH | 8% as effective as NADH | Escherichia coli |