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Literature summary for 1.1.1.95 extracted from

  • Pizer, L.I.; Sugimoto, E.
    3-Phosphoglycerate dehydrogenase (Escherichia coli) (1971), Methods Enzymol., 17B, 325-331.
No PubMed abstract available

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Escherichia coli

General Stability

General Stability Organism
dithiothreitol increases stability Escherichia coli

Inhibitors

Inhibitors Comment Organism Structure
Ag+ inhibition can be overcome by addition of dithiothreitol Escherichia coli
L-serine inhibition of enzyme from E. coli, Salmonella typhimurium and Haemophilus influenzae, not of mammalian enzyme, inhibition in both reaction directions Escherichia coli
Mercurials inhibition can be overcome by addition of dithiothreitol Escherichia coli

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
3-phosphohydroxypyruvate Km at pH 7.5 is lower than at pH 8.8 Escherichia coli
0.0078
-
NAD+ Km at pH 7.5 is lower than at pH 8.8 Escherichia coli
1.1
-
D-3-phosphoglycerate
-
Escherichia coli

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
163000
-
sedimentation equilibrium analysis Escherichia coli

Organism

Organism UniProt Comment Textmining
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-phospho-D-glycerate + NAD+
-
Escherichia coli 3-phosphohydroxypyruvate + NADH
-
?
3-phosphoglycerate + NAD+
-
Escherichia coli 3-phosphohydroxypyruvate + NADH
-
r

Subunits

Subunits Comment Organism
tetramer 4 * 40000-50000, sedimentation equilibrium in guanidine-HCl Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
initial rate maximum, but enzyme not stable Escherichia coli

Cofactor

Cofactor Comment Organism Structure
3-acetylpyridine-NADH 40% as effective as NADH Escherichia coli
deamino-NADH as effective as NADH Escherichia coli
NAD+
-
Escherichia coli
NADH
-
Escherichia coli
NADPH 8% as effective as NADH Escherichia coli