Protein Variants | Comment | Organism |
---|---|---|
additional information | existence of a naturally occurring feedback inhibition-resistant mutant | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2',5'-ADP | - |
Escherichia coli | |
3,4-dihydroxybutyl 1-phosphonate | competitive versus dihydroxyacetone phosphate, binding to a regulatory site | Escherichia coli | |
ADP | - |
Escherichia coli | |
ADP-ribose | - |
Escherichia coli | |
AMP | - |
Escherichia coli | |
ATP | - |
Escherichia coli | |
ethylene glycol phosphate | binding to the active site | Escherichia coli | |
NAD+ | - |
Escherichia coli | |
NADP+ | - |
Escherichia coli | |
NMN | - |
Escherichia coli | |
sn-glycerol-3-phosphate | the wild-type enzyme is feedback regulated, competitive versus dihydroxyacetone phosphate, binding to a regulatory site | Escherichia coli |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0037 | - |
NADPH | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
0.004 | - |
NADH | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
0.0041 | - |
NADPH | pH 7.4, 23°C, wild-type mutant enzyme | Escherichia coli | |
0.0045 | - |
NADH | pH 7.4, 23°C, wild-type mutant enzyme | Escherichia coli | |
0.005 | - |
reduced nicotinamide hypoxanthine dinucleotide | pH 7.4, 23°C, wild-type mutant enzyme and feedback-resistant mutant enzyme | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate + NAD(P)H | Escherichia coli | - |
sn-glycerol-3-phosphate + NAD(P)+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycerone phosphate + NAD(P)H | - |
Escherichia coli | sn-glycerol-3-phosphate + NAD(P)+ | - |
r | |
glycerone phosphate + NAD(P)H | i.e. dihydroxyacetone phosphate | Escherichia coli | sn-glycerol-3-phosphate + NAD(P)+ | product analysis | r | |
glycerone phosphate + NADH + H+ | - |
Escherichia coli | sn-glycerol 3-phosphate + NAD+ | - |
? | |
glycerone phosphate + reduced nicotinamide hypoxanthine dinucleotide | - |
Escherichia coli | sn-glycerol-3-phosphate + oxidized nicotinamide hypoxanthine dinucleotide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
sn-glycerol-3-phosphate dehydrogenase | - |
Escherichia coli |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
23 | - |
assay at | Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Escherichia coli | |
NADH | - |
Escherichia coli | |
NADP+ | B-type specificity of wild-type and feedback-resistant mutant enzyme | Escherichia coli | |
NADPH | B-type specificity of wild-type and feedback-resistant mutant enzyme | Escherichia coli | |
nicotinamide hypoxanthine dinucleotide | - |
Escherichia coli |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | product inhibition kinetics | Escherichia coli | |
0.1 | - |
ADP-ribose | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
0.19 | - |
NADP+ | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
0.2 | - |
NAD+ | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
0.21 | - |
ADP-ribose | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
0.22 | - |
NAD+ | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
0.25 | - |
NADP+ | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
0.7 | - |
ADP | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
0.8 | - |
ADP | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
1.4 | - |
ethylene glycol phosphate | pH 7.4, 23°C, wild-type and feedback-resistant mutant enzyme | Escherichia coli | |
1.4 | - |
ATP | pH 7.4, 23°C, wild-type enzyme and feedback-resistant mutant enzyme | Escherichia coli | |
1.8 | - |
2',5'-ADP | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
2 | - |
2',5'-ADP | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
4.8 | - |
AMP | pH 7.4, 23°C, feedback-resistant mutant enzyme | Escherichia coli | |
5 | - |
AMP | pH 7.4, 23°C, wild-type enzyme | Escherichia coli | |
10 | - |
NMN | pH 7.4, 23°C, wild-type enzyme and feedback-resistant mutant enzyme | Escherichia coli |