Inhibitors | Comment | Organism | Structure |
---|---|---|---|
5,5'-dithiobis-(2-nitrobenzoate) | - |
Thauera sp. | |
additional information | no inhibition by O2, EDTA, 2,2'bipyridy, pyrazole | Thauera sp. | |
p-chloromercuribenzoate | - |
Thauera sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NAD+ | - |
Thauera sp. | |
0.3 | - |
4-Hydroxybenzyl alcohol | - |
Thauera sp. | |
0.32 | - |
benzyl alcohol | - |
Thauera sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
4 * 40000, SDS-PAGE | Thauera sp. |
160000 | - |
gel filtration | Thauera sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
benzyl alcohol + NAD+ | Thauera sp. | - |
benzaldehyde + NADH | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera sp. | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.106 | - |
- |
Thauera sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-fluorobenzyl alcohol + NAD+ | - |
Thauera sp. | 2-fluorobenzaldehyde + NADH | - |
r | |
4-hydroxybenzyl alcohol + NAD+ | 125% of benzyl alcohol activity | Thauera sp. | 4-hydroxybenzaldehyde + NADH | - |
r | |
benzyl alcohol + NAD+ | - |
Thauera sp. | benzaldehyde + NADH | - |
r |
Subunits | Comment | Organism |
---|---|---|
tetramer | 4 * 40000, SDS-PAGE | Thauera sp. |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
85 | - |
benzyl alcohol | - |
Thauera sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
reduction of benzaldehyde | Thauera sp. |
9.2 | - |
benzyl alcohol + NAD+ | Thauera sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4.7 | 7.6 | less than half-maximal activity above and below, reduction of benzaldehyde | Thauera sp. |
8.5 | 9.7 | less than half-maximal activity above and below, oxidation of benzyl alcohol | Thauera sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Thauera sp. | |
NADH | - |
Thauera sp. |