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Literature summary for 1.1.1.90 extracted from

  • Curtis, A.J.; Shirk, M.C.; Fall, R.
    Allylic or benzylic stabilization is essential for catalysis by bacterial benzyl alcohol dehydrogenases (1999), Biochem. Biophys. Res. Commun., 259, 220-223.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudomonas putida
expression in Escherichia coli Acinetobacter calcoaceticus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.017
-
3-methyl-2-buten-1-ol
-
Acinetobacter calcoaceticus
0.046
-
benzyl alcohol
-
Acinetobacter calcoaceticus
0.11
-
3-methyl-2-buten-1-ol
-
Pseudomonas putida
0.13
-
benzyl alcohol
-
Pseudomonas putida

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
benzyl alcohol + NAD+ Pseudomonas putida
-
benzaldehyde + NADH
-
?
benzyl alcohol + NAD+ Acinetobacter calcoaceticus
-
benzaldehyde + NADH
-
?

Organism

Organism UniProt Comment Textmining
Acinetobacter calcoaceticus
-
-
-
Pseudomonas putida
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-perillyl alcohol + NAD+ 61% activity relative to benzyl alcohol Acinetobacter calcoaceticus (R)-perillaldehyde + NADH
-
?
(R)-perillyl alcohol + NAD+ 49% activity relative to benzyl alcohol Pseudomonas putida (R)-perillaldehyde + NADH
-
?
(S)-perillyl alcohol + NAD+ 85% activity relative to benzyl alcohol Acinetobacter calcoaceticus (S)-perillaldehyde + NADH
-
?
(S)-perillyl alcohol + NAD+ 68% activity relative to benzyl alcohol Pseudomonas putida (S)-perillaldehyde + NADH
-
?
3-methyl-2-buten-1-ol + NAD+ 210% activity relative to benzyl alcohol Acinetobacter calcoaceticus 3-methyl-2-buten-1-al + NADH
-
?
3-methyl-2-buten-1-ol + NAD+ 180% activity relative to benzyl alcohol Pseudomonas putida 3-methyl-2-buten-1-al + NADH
-
r
4-aminobenzyl alcohol + NAD+ 270% activity relative to benzyl alcohol Acinetobacter calcoaceticus 4-aminobenzaldehyde + NADH
-
r
4-aminobenzyl alcohol + NAD+ 280% activity relative to benzyl alcohol Pseudomonas putida 4-aminobenzaldehyde + NADH
-
r
benzyl alcohol + NAD+
-
Pseudomonas putida benzaldehyde + NADH
-
?
benzyl alcohol + NAD+
-
Acinetobacter calcoaceticus benzaldehyde + NADH
-
?
geraniol + NAD+ 68% activity relative to benzyl alcohol Pseudomonas putida geranial + NADH + H+
-
?
geraniol + NAD+ 63% activity relative to benzyl alcohol Acinetobacter calcoaceticus geranial + NADH + H+
-
?
nerol + NAD+ 25% activity relative to benzyl alcohol Acinetobacter calcoaceticus neral + NADH + H+
-
?
nerol + NAD+ 77% activity relative to benzyl alcohol Pseudomonas putida neral + NADH + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
18
-
benzyl alcohol
-
Pseudomonas putida
37
-
3-methyl-2-buten-1-ol
-
Acinetobacter calcoaceticus
41
-
3-methyl-2-buten-1-ol
-
Acinetobacter calcoaceticus
63
-
benzyl alcohol
-
Acinetobacter calcoaceticus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Pseudomonas putida
NAD+
-
Acinetobacter calcoaceticus
NADH
-
Pseudomonas putida
NADH
-
Acinetobacter calcoaceticus