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Literature summary for 1.1.1.9 extracted from

  • Mouro, A.; Santos, A.; Agnolo, D.; Gubert, G.; Bon, E.; Rosa, C.; Fonseca, C.; Stambuk, B.
    Combining xylose reductase from Spathaspora arborariae with xylitol dehydrogenase from Spathaspora passalidarum to promote xylose consumption and fermentation into xylitol by Saccharomyces cerevisiae (2020), Fermentation, 6, 72 .
No PubMed abstract available
Search for more literature for 1.1.1.9

Cloned(Commentary)

Cloned (Comment) Organism
gene SpXYL2.2, recombinant expression of the enzyme in Saccharomyces cerevisiae strain CEN.PK2-1C (MATa leu2-3112 ura3-52 trp1-289 his3-DELTA1 MAL2-8c SUC2), subcloning in Escherichia coli strain DH5alpha, co-expression with xylose reductase from Spathaspora arborariae Spathaspora passalidarum

Protein Variants

Protein Variants Comment Organism
additional information combination of xylose reductase from Spathaspora arborariae with xylitol dehydrogenase from Spathaspora passalidarum to promote xylose consumption and fermentation into xylitol in Saccharomyces cerevisiae. Recombinant co-expression of Spathaspora arborariae xylose reductase gene (SaXYL1) that accepts both NADH and NADPH as co-substrates, and of Spathaspora passalidarum strain UFMG-HM.19.1AT NADPH-dependent xylose reductase (SpXYL1.1 gene) or the SpXYL2.2 gene from Spathaspora passalidarum strain UFMG-CM-Y474 in a Saccharomyces cerevisiae strain overexpressing the native XKS1 gene encoding xylulokinase, as well as being deleted in the alkaline phosphatase encoded by the PHO13 gene. Strains expressing the Spathaspora enzymes consumes xylose with xylitol as the major fermentation product. Higher specific growth rates, xylose consumption, and xylitol volumetric productivities are obtained by the co-expression of the SaXYL1 and SpXYL2.2 genes, when compared with the co-expression of the NADPH-dependent SpXYL1.1 xylose reductase. During glucose-xylose co-fermentation by the strain with co-expression of the SaXYL1 and SpXYL2.2 genes, both ethanol and xylitol are produced efficiently. Method development and evaluation, detailed overview Spathaspora passalidarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Michaelis-Menten kinetics Spathaspora passalidarum
0.52
-
 NAD+ recombinant enzyme, pH 9.0, 35°C Spathaspora passalidarum
86
-
 xylitol recombinant enzyme, pH 9.0, 35°C Spathaspora passalidarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Spathaspora passalidarum
-
 D-xylulose + NADH + H+
-
 r
xylitol + NAD+ Spathaspora passalidarum UFMG-CM-Y474
-
 D-xylulose + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Spathaspora passalidarum
-
 isolated from rotting wood in Roraima, Brazil
-
Spathaspora passalidarum UFMG-CM-Y474
-
 isolated from rotting wood in Roraima, Brazil
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.003
-
 native enzyme, substrate xylose and NADP+, pH 9.0, 35°C Spathaspora passalidarum
1.29
-
 native enzyme, substrate xylitol and NAD+, pH 9.0, 35°C Spathaspora passalidarum
2.2
-
 recombinant enzyme expressed in Saccharomyces cerevisiae strain CEN.PK2-1C, substrate xylitol and NAD+, pH 9.0, 35°C Spathaspora passalidarum
2.95
-
 recombinant enzyme expressed in Saccharomyces cerevisiae strain CEN.PK2-1C, co-expressed with xylose reductase from Spathaspora arborariae, substrate xylitol and NAD+, pH 9.0, 35°C Spathaspora passalidarum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
xylitol + NAD+
-
 Spathaspora passalidarum D-xylulose + NADH + H+
-
 r
xylitol + NAD+
-
 Spathaspora passalidarum UFMG-CM-Y474 D-xylulose + NADH + H+
-
 r

Synonyms

Synonyms Comment Organism
SpXYL2.2
-
 Spathaspora passalidarum
XDH
-
 Spathaspora passalidarum
xylitol dehydrogenase
-
 Spathaspora passalidarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
 xylitol oxidation, assay at Spathaspora passalidarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9
-
 xylitol oxidation, assay at Spathaspora passalidarum

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Spathaspora passalidarum
NADH
-
 Spathaspora passalidarum