Cloned (Comment) | Organism |
---|---|
gene xyl2, DNA and amino acid sequence determination and analysis, cloning in Escherichia coli strain JM109, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) | Meyerozyma caribbica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Meyerozyma caribbica | |
iodoacetic acid | - |
Meyerozyma caribbica | |
o-phenanthroline | - |
Meyerozyma caribbica | |
Tiron | - |
Meyerozyma caribbica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.1 | - |
xylitol | recombinant enzyme, pH 7.0, 30°C | Meyerozyma caribbica | |
0.11 | - |
NAD+ | at pH 7.0 and 30°C | Meyerozyma caribbica | |
0.11 | - |
xylitol | native enzyme, pH 7.0, 30°C | Meyerozyma caribbica | |
15.4 | - |
xylitol | recombinant enzyme, pH 7.0, 30°C | Meyerozyma caribbica | |
15.8 | - |
ribitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
16 | - |
xylitol | native enzyme, pH 7.0, 30°C | Meyerozyma caribbica | |
16.1 | - |
xylitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
21.2 | - |
D-sorbitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
31.1 | - |
L-arabitol | at pH 7.0 and 30°C | Meyerozyma caribbica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | zinc metalloenzyme | Meyerozyma caribbica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
- |
Meyerozyma caribbica |
195000 | - |
gel filtration | Meyerozyma caribbica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | Meyerozyma caribbica | - |
D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | Meyerozyma caribbica | preferred substrate | D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | Meyerozyma caribbica 5XY2 | preferred substrate | D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | Meyerozyma caribbica 5XY2 | - |
D-xylulose + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Meyerozyma caribbica | - |
- |
- |
Meyerozyma caribbica | A0A1B4XTS0 | pentose-fermenting strain isolated from an alcohol fermentation starter in Thailand | - |
Meyerozyma caribbica 5XY2 | - |
- |
- |
Meyerozyma caribbica 5XY2 | A0A1B4XTS0 | pentose-fermenting strain isolated from an alcohol fermentation starter in Thailand | - |
Purification (Comment) | Organism |
---|---|
DEAEToyopearl 650 M column chromatography, butyl-Toyopearl 650 M column chromatography, MonoQ column chromatography, and Superdex 200 gel filtration | Meyerozyma caribbica |
native enzyme by ammonium sulfate fractionation, anion exchange chromatography, hydrophobic interation chromatography, and another different step of anion exchange chromatography, followed by gel filtration. Recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion exchange chromatography | Meyerozyma caribbica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | strain 5XY2 catabolize L-arabinose as well as D-glucose and D-xylose in fermentation | Meyerozyma caribbica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.08 | - |
crude enzyme, at pH 7.0 and 30°C | Meyerozyma caribbica |
2 | - |
purified recombinant enzyme, pH 7.0, 40°C | Meyerozyma caribbica |
4.9 | - |
after purification, at pH 7.0 and 30°C | Meyerozyma caribbica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-sorbitol + NAD+ | - |
Meyerozyma caribbica | D-fructose + NADH + H+ | - |
r | |
D-sorbitol + NAD+ | - |
Meyerozyma caribbica 5XY2 | D-fructose + NADH + H+ | - |
r | |
D-sorbitol + NAD+ | - |
Meyerozyma caribbica | ? + NADH + H+ | - |
? | |
D-sorbitol + NAD+ | - |
Meyerozyma caribbica 5XY2 | ? + NADH + H+ | - |
? | |
L-arabitol + NAD+ | the enzyme also has L-arabitol dehydrogenase activity (20.1% activity compared to xylitol) | Meyerozyma caribbica | L-xylulose + NADH + H+ | - |
? | |
L-arabitol + NAD+ | the enzyme also has L-arabitol dehydrogenase activity (20.1% activity compared to xylitol) | Meyerozyma caribbica 5XY2 | L-xylulose + NADH + H+ | - |
? | |
additional information | no activity with D-mannitol, erythritol, and D-arabitol. Very low activity with NADP+ | Meyerozyma caribbica | ? | - |
? | |
additional information | the enzyme exhibits broad specificity to polyols, such as xylitol, D-sorbitol, ribitol, and L-arabinitol. Xylitol is the preferred substrate, but native and recombinant enzyme McXDH exhibits relative activities toward L-arabinitol of approximately 20% that toward xylitol. No activity with D-mannitol, erythritol, and D-arabinitol | Meyerozyma caribbica | ? | - |
? | |
additional information | no activity with D-mannitol, erythritol, and D-arabitol. Very low activity with NADP+ | Meyerozyma caribbica 5XY2 | ? | - |
? | |
additional information | the enzyme exhibits broad specificity to polyols, such as xylitol, D-sorbitol, ribitol, and L-arabinitol. Xylitol is the preferred substrate, but native and recombinant enzyme McXDH exhibits relative activities toward L-arabinitol of approximately 20% that toward xylitol. No activity with D-mannitol, erythritol, and D-arabinitol | Meyerozyma caribbica 5XY2 | ? | - |
? | |
ribitol + NAD+ | - |
Meyerozyma caribbica | ? | - |
? | |
ribitol + NAD+ | - |
Meyerozyma caribbica 5XY2 | ? | - |
? | |
ribitol + NAD+ | reaction of EC 1.1.1.56, ribitol 2-dehydrogenase | Meyerozyma caribbica | D-ribulose + NADH + H+ | - |
? | |
ribitol + NAD+ | reaction of EC 1.1.1.56, ribitol 2-dehydrogenase | Meyerozyma caribbica 5XY2 | D-ribulose + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Meyerozyma caribbica | D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | preferred substrate | Meyerozyma caribbica | D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | preferred substrate | Meyerozyma caribbica 5XY2 | D-xylulose + NADH + H+ | - |
? | |
xylitol + NAD+ | - |
Meyerozyma caribbica 5XY2 | D-xylulose + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homopentamer | - |
Meyerozyma caribbica |
homopentamer | 5 * 39000, SDS-PAGE | Meyerozyma caribbica |
Synonyms | Comment | Organism |
---|---|---|
XDH | - |
Meyerozyma caribbica |
XYL2 | - |
Meyerozyma caribbica |
xylitol dehydrogenase | - |
Meyerozyma caribbica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Meyerozyma caribbica |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 55 | activity range, inactive at 60°C | Meyerozyma caribbica |
45 | 55 | the enzyme partially loses its activity at 45-55°C and also completely loses activity at temperatures greater than 60°C | Meyerozyma caribbica |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 45 | the enzyme exhibits activities of approximately 81% and 51% after being incubated at 30°C and 35°C, respectively, for 30 min. The enzyme completely loses its activity at temperatures greater than 45°C | Meyerozyma caribbica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.12 | - |
xylitol | recombinant enzyme, pH 7.0, 30°C | Meyerozyma caribbica | |
3.35 | - |
L-arabitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
4.5 | - |
ribitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
8.28 | - |
D-sorbitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
18 | - |
xylitol | at pH 7.0 and 30°C | Meyerozyma caribbica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | Meyerozyma caribbica |
9.5 | - |
- |
Meyerozyma caribbica |
9.5 | - |
native enzyme | Meyerozyma caribbica |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 11 | the enzyme lost its activity at pH lower than 4.0 and higher than 11.0, corresponding to NAD+ which is unstable at pH values greater than 10.5 | Meyerozyma caribbica |
4 | 11 | activity range, inactive above or below | Meyerozyma caribbica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Meyerozyma caribbica | |
NAD+ | preferred cofactor | Meyerozyma caribbica | |
NADP+ | low activity | Meyerozyma caribbica |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to a medium-chain dehydrogenase/reductase (MDR) superfamily and a subfamily of polyol dehydrogenase, PDH | Meyerozyma caribbica |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.11 | - |
L-arabitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
0.29 | - |
ribitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
0.39 | - |
D-sorbitol | at pH 7.0 and 30°C | Meyerozyma caribbica | |
1.12 | - |
xylitol | at pH 7.0 and 30°C | Meyerozyma caribbica |