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Literature summary for 1.1.1.9 extracted from
- Enhanced xylitol production: Expression of xylitol dehydrogenase from Gluconobacter oxydans and mixed culture of resting cell (2016), J. Biosci. Bioeng., 122, 257-262.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Escherichia coli BL21 cells | Gluconobacter oxydans |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 36.4 | - |
NADH | at pH 5.0 and 35°C | Gluconobacter oxydans |  |
| 43.9 | - |
D-xylulose | at pH 5.0 and 35°C | Gluconobacter oxydans | |
| 107.3 | - |
NAD+ | at pH 7.0 and 30°C | Gluconobacter oxydans | |
| 175.5 | - |
xylitol | at pH 7.0 and 30°C | Gluconobacter oxydans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 29000 | - |
x * 29000, SDS-PAGE | Gluconobacter oxydans |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-xylulose + NADH + H+ | Gluconobacter oxydans | 100% activity | xylitol + NAD+ | - |
r | |
| D-xylulose + NADH + H+ | Gluconobacter oxydans CGMCC 1.49 | 100% activity | xylitol + NAD+ | - |
r | |
| xylitol + NAD+ | Gluconobacter oxydans | 100% activity | D-xylulose + NADH + H+ | - |
r | |
| xylitol + NAD+ | Gluconobacter oxydans CGMCC 1.49 | 100% activity | D-xylulose + NADH + H+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Gluconobacter oxydans | - |
- |
- |
| Gluconobacter oxydans CGMCC 1.49 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-NTA column chromatography and Sephacryl S-300 gel filtration | Gluconobacter oxydans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-fructose + NADH + H+ | 46.4% activity compared to D-xylulose | Gluconobacter oxydans | sorbitol + NAD+ | - |
r | |
| D-fructose + NADH + H+ | 46.4% activity compared to D-xylulose | Gluconobacter oxydans CGMCC 1.49 | sorbitol + NAD+ | - |
r | |
| D-mannitol + NAD+ | 16.1% activity compared to xylitol | Gluconobacter oxydans | ? | - |
? | |
| D-mannitol + NAD+ | 16.1% activity compared to xylitol | Gluconobacter oxydans CGMCC 1.49 | ? | - |
? | |
| D-xylulose + NADH + H+ | 100% activity | Gluconobacter oxydans | xylitol + NAD+ | - |
r | |
| D-xylulose + NADH + H+ | 100% activity | Gluconobacter oxydans CGMCC 1.49 | xylitol + NAD+ | - |
r | |
| sorbitol + NAD+ | 153.9% activity compared to xylitol | Gluconobacter oxydans | D-fructose + NADH + H+ | - |
r | |
| sorbitol + NAD+ | 153.9% activity compared to xylitol | Gluconobacter oxydans CGMCC 1.49 | D-fructose + NADH + H+ | - |
r | |
| xylitol + NAD+ | 100% activity | Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
r | |
| xylitol + NAD+ | 100% activity | Gluconobacter oxydans CGMCC 1.49 | D-xylulose + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 29000, SDS-PAGE | Gluconobacter oxydans |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| XDH | - |
Gluconobacter oxydans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
optimum temperature for the oxidation of xylitol in the presence of coenzyme NAD+ | Gluconobacter oxydans |
| 35 | - |
optimum temperature for the for the reduction of D-xylulose in the presence of NADH | Gluconobacter oxydans |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 40 | the oxidation and reduction activities of the enzyme on the condition of temperatures ranging from 25°C to 40°C are over 80% | Gluconobacter oxydans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
optimum pH for the for the reduction of D-xylulose in the presence of NADH | Gluconobacter oxydans |
| 11 | - |
optimum pH for the oxidation of xylitol in the presence of coenzyme NAD+ | Gluconobacter oxydans |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Gluconobacter oxydans | |
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Release 2023.1 (January 2023)
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