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Literature summary for 1.1.1.9 extracted from

  • Zhang, J.; Li, S.; Xu, H.; Zhou, P.; Zhang, L.; Ouyang, P.
    Purification of xylitol dehydrogenase and improved production of xylitol by increasing XDH activity and NADH supply in Gluconobacter oxydans (2013), J. Agric. Food Chem., 61, 2861-2867.
No PubMed abstract available

Application

Application Comment Organism
synthesis Gluconobacter oxydans strain NH-10 is useful for production of xylitol from D-arabitol via D-xylulose Gluconobacter oxydans

Cloned(Commentary)

Cloned (Comment) Organism
gene xdhA, coexpression of gene xdhA and cofactor regeneration enzyme, i.e. glucose dehydrogenase gene gdh from Bacillus subtilis, in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 Gluconobacter oxydans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
xylitol + NAD+ Gluconobacter oxydans
-
D-xylulose + NADH + H+
-
r
xylitol + NAD+ Gluconobacter oxydans NH-10
-
D-xylulose + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Gluconobacter oxydans
-
isolated from soil, China
-
Gluconobacter oxydans NH-10
-
isolated from soil, China
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 469fold from sstrain NH-10 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, and gel filtration Gluconobacter oxydans

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.76
-
purified native enzyme, pH 11.0, 30°C, substrate meso-erythritol Gluconobacter oxydans
6.74
-
purified native enzyme, pH 11.0, 30°C, substrate D-arabitol Gluconobacter oxydans
12.89
-
purified native enzyme, pH 11.0, 30°C, substrate D-mannitol Gluconobacter oxydans
102.5
-
purified native enzyme, pH 11.0, 30°C, substrate xylitol Gluconobacter oxydans
156.1
-
purified native enzyme, pH 11.0, 30°C, substrate D-sorbitol Gluconobacter oxydans
180.4
-
purified native enzyme, pH 5.0, 30°C, substrate D-fructose Gluconobacter oxydans
564
-
purified native enzyme, pH 5.0, 30°C, substrate D-xylulose Gluconobacter oxydans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabitol + NAD+
-
Gluconobacter oxydans ? + NADH + H+
-
r
D-arabitol + NAD+
-
Gluconobacter oxydans NH-10 ? + NADH + H+
-
r
D-mannitol + NAD+
-
Gluconobacter oxydans D-fructose + NADH + H+
-
r
D-mannitol + NAD+
-
Gluconobacter oxydans NH-10 D-fructose + NADH + H+
-
r
D-sorbitol + NAD+
-
Gluconobacter oxydans L-sorbose + NADH + H+
-
r
D-sorbitol + NAD+
-
Gluconobacter oxydans NH-10 L-sorbose + NADH + H+
-
r
D-xylulose + NAD+
-
Gluconobacter oxydans xylitol + NADH + H+
-
r
meso-erythritol + NAD+
-
Gluconobacter oxydans ? + NADH + H+
-
r
meso-erythritol + NAD+
-
Gluconobacter oxydans NH-10 ? + NADH + H+
-
r
xylitol + NAD+
-
Gluconobacter oxydans D-xylulose + NADH + H+
-
r
xylitol + NAD+
-
Gluconobacter oxydans NH-10 D-xylulose + NADH + H+
-
r

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
reduction reaction Gluconobacter oxydans
35
-
oxidation reaction Gluconobacter oxydans

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 55 activity range, profile overview Gluconobacter oxydans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.5
-
reduction reaction Gluconobacter oxydans
10.5 11 oxidation reaction Gluconobacter oxydans

pH Range

pH Minimum pH Maximum Comment Organism
4 8 reduction reaction, activity range Gluconobacter oxydans
9 12 oxidation reaction, activity range Gluconobacter oxydans

Cofactor

Cofactor Comment Organism Structure
additional information XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity Gluconobacter oxydans
NAD+
-
Gluconobacter oxydans
NADH
-
Gluconobacter oxydans

General Information

General Information Comment Organism
metabolism bioproduction pathway of xylitol, overview Gluconobacter oxydans
additional information XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity limiting the the overall conversion process, improvement by recombinant expression of enzyme and glucose dehydrogenase cofactor regeneration enzyme Gluconobacter oxydans