Application | Comment | Organism |
---|---|---|
synthesis | Gluconobacter oxydans strain NH-10 is useful for production of xylitol from D-arabitol via D-xylulose | Gluconobacter oxydans |
Cloned (Comment) | Organism |
---|---|
gene xdhA, coexpression of gene xdhA and cofactor regeneration enzyme, i.e. glucose dehydrogenase gene gdh from Bacillus subtilis, in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain JM109 | Gluconobacter oxydans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylitol + NAD+ | Gluconobacter oxydans | - |
D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | Gluconobacter oxydans NH-10 | - |
D-xylulose + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Gluconobacter oxydans | - |
isolated from soil, China | - |
Gluconobacter oxydans NH-10 | - |
isolated from soil, China | - |
Purification (Comment) | Organism |
---|---|
native enzyme 469fold from sstrain NH-10 by ammonium sulfate fractionation, two different steps of anion exchange chromatography, and gel filtration | Gluconobacter oxydans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.76 | - |
purified native enzyme, pH 11.0, 30°C, substrate meso-erythritol | Gluconobacter oxydans |
6.74 | - |
purified native enzyme, pH 11.0, 30°C, substrate D-arabitol | Gluconobacter oxydans |
12.89 | - |
purified native enzyme, pH 11.0, 30°C, substrate D-mannitol | Gluconobacter oxydans |
102.5 | - |
purified native enzyme, pH 11.0, 30°C, substrate xylitol | Gluconobacter oxydans |
156.1 | - |
purified native enzyme, pH 11.0, 30°C, substrate D-sorbitol | Gluconobacter oxydans |
180.4 | - |
purified native enzyme, pH 5.0, 30°C, substrate D-fructose | Gluconobacter oxydans |
564 | - |
purified native enzyme, pH 5.0, 30°C, substrate D-xylulose | Gluconobacter oxydans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-arabitol + NAD+ | - |
Gluconobacter oxydans | ? + NADH + H+ | - |
r | |
D-arabitol + NAD+ | - |
Gluconobacter oxydans NH-10 | ? + NADH + H+ | - |
r | |
D-mannitol + NAD+ | - |
Gluconobacter oxydans | D-fructose + NADH + H+ | - |
r | |
D-mannitol + NAD+ | - |
Gluconobacter oxydans NH-10 | D-fructose + NADH + H+ | - |
r | |
D-sorbitol + NAD+ | - |
Gluconobacter oxydans | L-sorbose + NADH + H+ | - |
r | |
D-sorbitol + NAD+ | - |
Gluconobacter oxydans NH-10 | L-sorbose + NADH + H+ | - |
r | |
D-xylulose + NAD+ | - |
Gluconobacter oxydans | xylitol + NADH + H+ | - |
r | |
meso-erythritol + NAD+ | - |
Gluconobacter oxydans | ? + NADH + H+ | - |
r | |
meso-erythritol + NAD+ | - |
Gluconobacter oxydans NH-10 | ? + NADH + H+ | - |
r | |
xylitol + NAD+ | - |
Gluconobacter oxydans | D-xylulose + NADH + H+ | - |
r | |
xylitol + NAD+ | - |
Gluconobacter oxydans NH-10 | D-xylulose + NADH + H+ | - |
r |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
reduction reaction | Gluconobacter oxydans |
35 | - |
oxidation reaction | Gluconobacter oxydans |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 55 | activity range, profile overview | Gluconobacter oxydans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
reduction reaction | Gluconobacter oxydans |
10.5 | 11 | oxidation reaction | Gluconobacter oxydans |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 8 | reduction reaction, activity range | Gluconobacter oxydans |
9 | 12 | oxidation reaction, activity range | Gluconobacter oxydans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity | Gluconobacter oxydans | |
NAD+ | - |
Gluconobacter oxydans | |
NADH | - |
Gluconobacter oxydans |
General Information | Comment | Organism |
---|---|---|
metabolism | bioproduction pathway of xylitol, overview | Gluconobacter oxydans |
additional information | XDH depends exclusively on NAD+/NADH as cofactors with a relatively low activity limiting the the overall conversion process, improvement by recombinant expression of enzyme and glucose dehydrogenase cofactor regeneration enzyme | Gluconobacter oxydans |