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Literature summary for 1.1.1.9 extracted from
- Purification and properties of xylitol dehydrogenase from the xylose-fermenting yeast Candida shehatae (1990), Appl. Biochem. Biotechnol., 26, 197-206.
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.037 | - |
NADH | - |
Scheffersomyces shehatae |  |
| 0.16 | - |
D-sorbitol | - |
Scheffersomyces shehatae | |
| 13.8 | - |
xylulose | - |
Scheffersomyces shehatae | |
| 18.5 | - |
xylitol | - |
Scheffersomyces shehatae | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cytoplasm | - |
Scheffersomyces shehatae | 5737 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 40000 | - |
2 * 40000, SDS-PAGE | Scheffersomyces shehatae |
| 82000 | - |
gel filtration | Scheffersomyces shehatae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| Xylitol + NAD+ | Scheffersomyces shehatae | inducible pathway of xylose catabolism | ? | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Scheffersomyces shehatae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Scheffersomyces shehatae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 15.95 | - |
- |
Scheffersomyces shehatae |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, stable with 50% v/v glycerol | Scheffersomyces shehatae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| D-ribitol + NAD+ | - |
Scheffersomyces shehatae | D-ribulose + NADH | - |
r | |
| D-ribulose + NADH | - |
Scheffersomyces shehatae | D-ribitol + NAD+ | - |
r | |
| D-sorbitol + NAD+ | oxidation at about 45% | Scheffersomyces shehatae | D-fructose + NADH + H+ | - |
? | |
| D-xylulose + NADH + H+ | - |
Scheffersomyces shehatae | xylitol + NAD+ | - |
r | |
| additional information | no oxidation of ribitol | Scheffersomyces shehatae | ? | - |
? | |
| additional information | no oxidation of mannitol | Scheffersomyces shehatae | ? | - |
? | |
| Xylitol + NAD+ | inducible pathway of xylose catabolism | Scheffersomyces shehatae | ? | - |
r | |
| xylitol + NAD+ | - |
Scheffersomyces shehatae | D-xylulose + NADH + H+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| dimer | 2 * 40000, SDS-PAGE | Scheffersomyces shehatae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
xylitol formation | Scheffersomyces shehatae |
| 8.6 | - |
D-xylulose formation | Scheffersomyces shehatae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activity with NADP+ | Scheffersomyces shehatae | |
| additional information | at high concentrations of xylitol 1.5% as effective as NAD+ | Scheffersomyces shehatae | |
| additional information | very low activity with NADPH | Scheffersomyces shehatae | |
| NAD+ | - |
Scheffersomyces shehatae | |
| NADH | - |
Scheffersomyces shehatae | |
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