Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Delftia acidovorans |
Crystallization (Comment) | Organism |
---|---|
apoenzyme, sitting drop vapor diffusion method, using 100 mM Bis-Tris (pH 5.5), 600 mM lithium sulfate, and 18% (w/v) PEG 3350. Mevalonate-bound enzyme, sitting drop vapor diffusion method, using 100 mM Bis-Tris (pH 6.5), 600 mM lithium sulfate, and 19% (w/v) PEG 3350. NADH-bound enzyme, sitting drop vapor diffusion method, using 100 mM sodium citrate (pH 5.0), 500 mM ammonium sulfate, and 600 mM lithium sulfate | Delftia acidovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
citrate | competitive inhibition with respect to 3-hydroxy-3-methylglutaryl-CoA | Delftia acidovorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00734 | - |
3-hydroxy-3-methylglutaryl-CoA | at pH 7.4, temperature not specified in the publication | Delftia acidovorans | |
0.0285 | - |
NADH | at pH 7.4, temperature not specified in the publication | Delftia acidovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | Delftia acidovorans | - |
(R)-mevalonate + CoA + 2 NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Delftia acidovorans | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography | Delftia acidovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ | - |
Delftia acidovorans | (R)-mevalonate + CoA + 2 NAD+ | - |
? | |
3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ | extremely low activity with NADPH | Delftia acidovorans | (R)-mevalonate + CoA + 2 NADP+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3-hydroxy-3-methylglutaryl coenzyme A reductase | - |
Delftia acidovorans |
HMG-CoA reductase | - |
Delftia acidovorans |
HMGR | - |
Delftia acidovorans |
mvaA | - |
Delftia acidovorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
16.6 | - |
NADH | at pH 7.4, temperature not specified in the publication | Delftia acidovorans | |
22.3 | - |
3-hydroxy-3-methylglutaryl-CoA | at pH 7.4, temperature not specified in the publication | Delftia acidovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADH-specific enzyme | Delftia acidovorans |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
170 | - |
citrate | at pH 7.4, temperature not specified in the publication | Delftia acidovorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
582 | - |
NADH | at pH 7.4, temperature not specified in the publication | Delftia acidovorans | |
3040 | - |
3-hydroxy-3-methylglutaryl-CoA | at pH 7.4, temperature not specified in the publication | Delftia acidovorans |