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Literature summary for 1.1.1.88 extracted from

  • Ragwan, E.R.; Arai, E.; Kung, Y.
    New Crystallographic snapshots of large domain movements in bacterial 3-hydroxy-3-methylglutaryl coenzyme A reductase (2018), Biochemistry, 57, 5715-5725 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Delftia acidovorans

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme, sitting drop vapor diffusion method, using 100 mM Bis-Tris (pH 5.5), 600 mM lithium sulfate, and 18% (w/v) PEG 3350. Mevalonate-bound enzyme, sitting drop vapor diffusion method, using 100 mM Bis-Tris (pH 6.5), 600 mM lithium sulfate, and 19% (w/v) PEG 3350. NADH-bound enzyme, sitting drop vapor diffusion method, using 100 mM sodium citrate (pH 5.0), 500 mM ammonium sulfate, and 600 mM lithium sulfate Delftia acidovorans

Inhibitors

Inhibitors Comment Organism Structure
citrate competitive inhibition with respect to 3-hydroxy-3-methylglutaryl-CoA Delftia acidovorans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00734
-
3-hydroxy-3-methylglutaryl-CoA at pH 7.4, temperature not specified in the publication Delftia acidovorans
0.0285
-
NADH at pH 7.4, temperature not specified in the publication Delftia acidovorans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+ Delftia acidovorans
-
(R)-mevalonate + CoA + 2 NAD+
-
?

Organism

Organism UniProt Comment Textmining
Delftia acidovorans
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Delftia acidovorans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
Delftia acidovorans (R)-mevalonate + CoA + 2 NAD+
-
?
3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ extremely low activity with NADPH Delftia acidovorans (R)-mevalonate + CoA + 2 NADP+
-
?

Synonyms

Synonyms Comment Organism
3-hydroxy-3-methylglutaryl coenzyme A reductase
-
Delftia acidovorans
HMG-CoA reductase
-
Delftia acidovorans
HMGR
-
Delftia acidovorans
mvaA
-
Delftia acidovorans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
16.6
-
NADH at pH 7.4, temperature not specified in the publication Delftia acidovorans
22.3
-
3-hydroxy-3-methylglutaryl-CoA at pH 7.4, temperature not specified in the publication Delftia acidovorans

Cofactor

Cofactor Comment Organism Structure
NADH NADH-specific enzyme Delftia acidovorans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
170
-
citrate at pH 7.4, temperature not specified in the publication Delftia acidovorans

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
582
-
NADH at pH 7.4, temperature not specified in the publication Delftia acidovorans
3040
-
3-hydroxy-3-methylglutaryl-CoA at pH 7.4, temperature not specified in the publication Delftia acidovorans