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Literature summary for 1.1.1.88 extracted from

  • Bensch, W.R.; Rodwell, V.W.
    Purification and properties of 3-hydroxy-3-methylglutaryl coenzyme A reductase from Pseudomonas (1970), J. Biol. Chem., 245, 3755-3762.
    View publication on PubMed
Search for more literature for 1.1.1.88

General Stability

General Stability Organism
dithiothreitol causes loss of activity Pseudomonas mevalonii
EDTA causes loss of activity Pseudomonas mevalonii

Inhibitors

Inhibitors Comment Organism Structure
3-hydroxy-3-methylglutarate competitive inhibitor Pseudomonas mevalonii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.032
-
 NADH reductive deacylation to mevalonate Pseudomonas mevalonii
0.039
-
 CoA oxidative acylation of mevalonate Pseudomonas mevalonii
0.046
-
 CoA mevaldate oxidation Pseudomonas mevalonii
0.05
-
 DL-3-hydroxy-3-methylglutaryl-CoA reductive deacylation to mevalonate Pseudomonas mevalonii
0.06
-
 NAD+ mevaldate oxidation Pseudomonas mevalonii
0.27
-
 NAD+ oxidative acylation of mevalonate Pseudomonas mevalonii
0.3
-
 mevalonate oxidative acylation of mevalonate Pseudomonas mevalonii
0.36
-
 NADH mevaldate reduction to mevalonate Pseudomonas mevalonii
0.61
-
 DL-mevaldate mevaldate oxidation Pseudomonas mevalonii
0.61
-
 DL-mevaldate oxidative acylation of DL-mevaldate Pseudomonas mevalonii
9
-
 DL-mevaldate mevaldate reduction to mevalonate Pseudomonas mevalonii
9
-
 DL-mevaldate DL-mevaldate reduction Pseudomonas mevalonii

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Pseudomonas mevalonii 5829
-

Organism

Organism UniProt Comment Textmining
Pseudomonas mevalonii
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-mevalonate + CoA + 2 NAD+
-
 Pseudomonas mevalonii (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADH + 2 H+
-
 r
3-hydroxy-3-methylglutaryl-CoA + NADH + H+
-
 Pseudomonas mevalonii mevalonate + CoA + NAD+
-
 ?
DL-mevaldate + CoA + NAD+
-
 Pseudomonas mevalonii 3-hydroxy-3-methylglutaryl-CoA NADH + H+
-
 r

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
9.2 9.6 oxidative acylation of mevalonate Pseudomonas mevalonii

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Pseudomonas mevalonii
NADH reverse reaction Pseudomonas mevalonii