Literature summary for 1.1.1.87 extracted from

Nango, E.; Yamamoto, T.; Kumasaka, T.; Eguchi, T.
Structure of Thermus thermophilus homoisocitrate dehydrogenase in complex with a designed inhibitor (2011), J. Biochem., 150, 607-614.

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme in binary complex with inhibitor (2S,3S)-thiahomoisocitrate, sitting drop vapour diffusion method, 0.00 ml of 8.7 mg/ml protein in 5 mM Tris-HCl, pH 7.8. 0.8 mM inhibitor, and 1.7 mM NAD, is mixed with 0.002 ml of reservoir solution containing 40% 2-methyl-2,4-pentandiol and 100 mM citrate pH 4.85, eqilibration against 1 ml resetvoir solution, X-ray diffrcation structure determination and analysis at 2.6 A resolution, molecular replacement	Thermus thermophilus

Crystallization (Comment)

Organism

enzyme in binary complex with inhibitor (2S,3S)-thiahomoisocitrate, sitting drop vapour diffusion method, 0.00 ml of 8.7 mg/ml protein in 5 mM Tris-HCl, pH 7.8. 0.8 mM inhibitor, and 1.7 mM NAD, is mixed with 0.002 ml of reservoir solution containing 40% 2-methyl-2,4-pentandiol and 100 mM citrate pH 4.85, eqilibration against 1 ml resetvoir solution, X-ray diffrcation structure determination and analysis at 2.6 A resolution, molecular replacement

Thermus thermophilus

Inhibitors

Inhibitors	Comment	Organism	Structure
(2S,3S)-(-)-3-methylmercaptomalic acid	-	Thermus thermophilus
(2S,3S)-thiahomoisocitrate	interacts through hydrogen bonding to Arg 118, Tyr 125 and Lys 171 in the active site, enzyme binding structure, overview	Thermus thermophilus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
7.5	-	(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate	pH 7.8, 60°C	Thermus thermophilus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	essentially required	Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+	Thermus thermophilus	-	2-oxoadipate + CO2 + NADH + H+	-	?

Organism

Organism	UniProt	Comment	Textmining
Thermus thermophilus	Q5SIJ1	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+	-	Thermus thermophilus	2-oxoadipate + CO2 + NADH + H+	-	?
(1R,2S)-1-hydroxybutane-1,2,4-tricarboxylate + NAD+	i.e. (2R,3S)-homoisocitrate	Thermus thermophilus	2-oxoadipate + CO2 + NADH + H+	-	?

Synonyms

Synonyms	Comment	Organism
HICDH	-	Thermus thermophilus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
60	-	assay at	Thermus thermophilus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.8	-	assay at	Thermus thermophilus

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Thermus thermophilus

Ki Value [mM]

Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
0.013	-	(2S,3S)-thiahomoisocitrate	pH 7.8, 60°C	Thermus thermophilus

General Information

General Information	Comment	Organism
metabolism	homoisocitrate dehydrogenase is involved in the alpha-aminoadipate pathway of lysine biosynthesis	Thermus thermophilus