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Literature summary for 1.1.1.87 extracted from

  • Yamamoto, T.; Miyazaki, K.; Eguchi, T.
    Substrate specificity analysis and inhibitor design of homoisocitrate dehydrogenase (2007), Bioorg. Med. Chem., 15, 1346-1355.
    View publication on PubMed
Search for more literature for 1.1.1.87

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
(R,E)-(3-carboxypropylidene)malic acid competitive Deinococcus radiodurans
(R,E)-(3-carboxypropylidene)malic acid competitive Saccharomyces cerevisiae
(R,E)-(3-hydroxypropylidene)malic acid competitive Deinococcus radiodurans
(R,E)-(3-hydroxypropylidene)malic acid competitive Saccharomyces cerevisiae
(R,Z)-(3-carboxypropylidene)malic acid competitive Deinococcus radiodurans
(R,Z)-(3-carboxypropylidene)malic acid competitive Saccharomyces cerevisiae
(R,Z)-(3-hydroxypropylidene)malic acid competitive Deinococcus radiodurans
(R,Z)-(3-hydroxypropylidene)malic acid competitive Saccharomyces cerevisiae
3-vinylmalate
-
 Deinococcus radiodurans
additional information synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview Deinococcus radiodurans
additional information synthesis and inhibition potency of substrate analogue inhibitors derived from 3-hydroxyalkylidene- and 3-carboxyalkylidenemalate derivatives, overview, no inhibition by 3-vinylmalate Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.018
-
 homoisocitrate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.083
-
 3-vinylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.211
-
 homoisocitrate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.29
-
 (2R,3S)-3-propylmalic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.291
-
 isocitrate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.41
-
 (2R,3S)-3-allylmalic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.44
-
 (2R,3S)-3-(4-hydroxybutyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.47
-
 (2R,3S)-3-(4-hydroxybutyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.51
-
 (2R,3S)-3-(3-hydroxypropyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.58
-
 (2R,3S)-3-(3-hydroxypropyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.59
-
 (2R,3S)-3-(2-hydroxyethyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.6
-
 ethylmalate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.78
-
 (2R,3S)-3-propylmalic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1
-
 ethylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
1.33
-
 isopropylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
2.1
-
 (2R,3S)-3-(2-hydroxyethyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
2.7
-
 (2R,3S)-3-(4-pentenyl)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
2.8
-
 (2R,3S)-3-allylmalic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
3.4
-
 3-vinylmalate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
9.4
-
 (2R,3S)-3-(4-pentenyl)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae
Mg2+
-
 Deinococcus radiodurans

Organism

Organism UniProt Comment Textmining
Deinococcus radiodurans
-
-
-
Saccharomyces cerevisiae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from Escherichia coli by anion exchange chromatography and gel filtration Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 substrate specificity, recombinant enzyme, overview Saccharomyces cerevisiae
additional information
-
 substrate specificity, recombinant enzyme, overview Deinococcus radiodurans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
(2R,3S)-3-(2-hydroxyethyl)malate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
(2R,3S)-3-(3-aminopropyl)malate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
(2R,3S)-3-(3-hydroxypropyl)malate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
(2R,3S)-3-(4-hydroxybutyl)malate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
(2R,3S)-3-(4-pentenyl)malic acid + NAD+
-
 Deinococcus radiodurans ?
-
 ?
(2R,3S)-3-allylmalic acid + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
(2R,3S)-3-allylmalic acid + NAD+
-
 Deinococcus radiodurans ?
-
 ?
(2R,3S)-3-propylmalic acid + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
(2R,3S)-3-propylmalic acid + NAD+
-
 Deinococcus radiodurans ?
-
 ?
3-vinylmalate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
3-vinylmalate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
ethylmalate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
ethylmalate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
homoisocitrate + NAD+
-
 Saccharomyces cerevisiae ?
-
 ?
homoisocitrate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
isocitrate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
isopropylmalate + NAD+
-
 Deinococcus radiodurans ?
-
 ?
additional information no activity with isocitrate, isopropylmalate, and tartrate Saccharomyces cerevisiae ?
-
 ?
additional information no activity with tartrate Deinococcus radiodurans ?
-
 ?

Synonyms

Synonyms Comment Organism
HICDH
-
 Saccharomyces cerevisiae
HICDH
-
 Deinococcus radiodurans

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
28
-
 assay at Deinococcus radiodurans
36
-
 assay at Saccharomyces cerevisiae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.14
-
 (2R,3S)-3-(4-pentenyl)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.34
-
 (2R,3S)-3-allylmalic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.37
-
 isopropylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.5
-
 3-vinylmalate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.61
-
 ethylmalate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.7
-
 (2R,3S)-3-propylmalic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.77
-
 3-vinylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.78
-
 (2R,3S)-3-(2-hydroxyethyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
2 8 (2R,3S)-3-(4-hydroxybutyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
2.1
-
 (2R,3S)-3-(4-hydroxybutyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
3
-
 (2R,3S)-3-(4-pentenyl)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
4.8
-
 (2R,3S)-3-(3-hydroxypropyl)malate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
17
-
 homoisocitrate pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
25
-
 (2R,3S)-3-(2-hydroxyethyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
29
-
 (2R,3S)-3-(3-hydroxypropyl)malate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
37
-
 (2R,3S)-3-allylmalic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
43
-
 isocitrate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
43
-
 ethylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
46
-
 homoisocitrate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
53
-
 (2R,3S)-3-propylmalic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.8
-
 assay at Saccharomyces cerevisiae
7.8
-
 assay at Deinococcus radiodurans

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Saccharomyces cerevisiae
NAD+
-
 Deinococcus radiodurans

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.072
-
 (R,Z)-(3-carboxypropylidene)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.088
-
 3-vinylmalate pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.26
-
 (R,Z)-(3-carboxypropylidene)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
0.51
-
 (R,Z)-(3-hydroxypropylidene)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
0.79
-
 (R,E)-(3-carboxypropylidene)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
1.4
-
 (R,E)-(3-hydroxypropylidene)malic acid pH 7.8, 36°C, recombinant enzyme Saccharomyces cerevisiae
3.1
-
 (R,E)-(3-carboxypropylidene)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
5.2
-
 (R,E)-(3-hydroxypropylidene)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans
15.3
-
 (R,Z)-(3-hydroxypropylidene)malic acid pH 7.8, 28°C, recombinant enzyme Deinococcus radiodurans