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Literature summary for 1.1.1.87 extracted from

  • Miyazaki, J.; Kobashi, N.; Nishiyama, M.; Yamane, H.
    Characterization of homoisocitrate dehydrogenase involved in lysine biosynthesis of an extremely thermophilic bacterium, Thermus thermophilus HB27, and evolutionary implication of beta-decarboxylating dehydrogenase (2003), J. Biol. Chem., 278, 1864-1871.
    View publication on PubMed
Search for more literature for 1.1.1.87

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) Thermus thermophilus

Protein Variants

Protein Variants Comment Organism
R85V complete loss of activity with isocitrate, significant activity with 3-isopropylmalate, no effect on activity with homoisocitrate Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.4
-
 1-hydroxy-1,2,3-propanetricarboxylate purified enzyme, pH 8.0, 60°C Thermus thermophilus
7.5
-
 1-hydroxy-1,2,4-butanetricarboxylate purified enzyme, pH 8.0, 60°C Thermus thermophilus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Thermus thermophilus involved in lysine biosynthesis through alpha-aminoadipate 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 involved in lysine biosynthesis through alpha-aminoadipate 2-oxoadipate + CO2 + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-
Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Thermus thermophilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
564
-
 purified enzyme, pH 8.0, 60°C, with 1-hydroxy-1,2,4-butanetricarboxylate as substrate Thermus thermophilus
8579
-
 purified enzyme, pH 8.0, 60°C, with 1-hydroxy-1,2,3-propanetricarboxylate as substrate Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxy-1,2,3-propanetricarboxylate + NAD+ more effective substrate than 1-hydroxy-1,2,4-butanetricarboxylate Thermus thermophilus ?
-
 ?
1-hydroxy-1,2,3-propanetricarboxylate + NAD+ more effective substrate than 1-hydroxy-1,2,4-butanetricarboxylate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Thermus thermophilus 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ involved in lysine biosynthesis through alpha-aminoadipate Thermus thermophilus 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ involved in lysine biosynthesis through alpha-aminoadipate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 2-oxoadipate + CO2 + NADH
-
 ?
additional information no activity with 3-isopropylmalate Thermus thermophilus ?
-
 ?
additional information no activity with 3-isopropylmalate Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039 ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
90
-
 half life: 16.7 h Thermus thermophilus
93.6
-
 melting point Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
171
-
 1-hydroxy-1,2,4-butanetricarboxylate purified enzyme, pH 8.0, 60°C Thermus thermophilus
171
-
 1-hydroxy-1,2,3-propanetricarboxylate purified enzyme, pH 8.0, 60°C Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermus thermophilus