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Literature summary for 1.1.1.87 extracted from
- Homoisocitric dehydrogenase from yeast (1970), Arch. Biochem. Biophys., 141, 499-510.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2-oxoadipate | required for reductive carboxylation | Saccharomyces cerevisiae |  |
| NaHCO3-CO2 | required for reductive carboxylation | Saccharomyces cerevisiae | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| homoisocitrate | 1.2 mM | Saccharomyces cerevisiae | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.01 | - |
homoisocitrate | value below | Saccharomyces cerevisiae | |
| 0.065 | - |
NADH | - |
Saccharomyces cerevisiae | |
| 0.33 | - |
NAD+ | - |
Saccharomyces cerevisiae | |
| 1.5 | - |
alpha-ketoadipate | - |
Saccharomyces cerevisiae | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | required for optimal activity | Saccharomyces cerevisiae | |
| Mg2+ | stimulation of oxidative decarboxylation, required for reductive carboxylation | Saccharomyces cerevisiae | |
| Mn2+ | stimulation of oxidative decarboxylation | Saccharomyces cerevisiae | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
gel filtration | Saccharomyces cerevisiae |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | Saccharomyces cerevisiae | - |
2-oxoadipate + CO2 + NADH | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Saccharomyces cerevisiae | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Saccharomyces cerevisiae |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.8 | - |
- |
Saccharomyces cerevisiae |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | - |
Saccharomyces cerevisiae | 2-oxoadipate + CO2 + NADH | - |
r | |
| 1-hydroxy-1,2,4-butanetricarboxylate + NAD+ | i.e. homoisocitrate | Saccharomyces cerevisiae | 2-oxoadipate + CO2 + NADH | - |
r | |
| additional information | catalyzes keto-enol tautomerization of tritiated alpha-ketoadipate, not: ethanol, isocitrate, malate, glutamate | Saccharomyces cerevisiae | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Saccharomyces cerevisiae |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
reductive carboxylation | Saccharomyces cerevisiae |
| 8.3 | 8.8 | oxidative decarboxylation | Saccharomyces cerevisiae |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.1 | 7.7 | pH 6.1: about 60% of activity maximum, pH 7.7: about 50% of activity maximum, reductive carboxylation | Saccharomyces cerevisiae |
| 7.4 | 9.3 | pH 7.4: about 50% of activity maximum, pH 9.3: about 90% of activity maximum, oxidative decarboxylation | Saccharomyces cerevisiae |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NAD+ | - |
Saccharomyces cerevisiae | |
| NADH | - |
Saccharomyces cerevisiae | |
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Release 2023.1 (January 2023)
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