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Literature summary for 1.1.1.87 extracted from

  • Garrad, R.C.; Bhattacharjee, J.K.
    Lysine biosynthesis in selected pathogenic fungi: characterization of lysine auxotrophs and the cloned LYS1 gene of Candida albicans (1992), J. Bacteriol., 174, 7379-7384.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.87

Application

Application Comment Organism
medicine detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo Candida albicans
medicine detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo Cryptococcus neoformans
medicine detection of unique gene LYS1 for rapid identification of pathogenic fungi, LYS1 gene is a possible target for selective inhibition of growth of pathogenic fungi in vivo Aspergillus fumigatus

Cloned(Commentary)

Cloned (Comment) Organism
LYS1 gene codes for the alpha-aminoadipate pathway enzymes in fungi, can complement LYS1-deficient mutants of Candida albicans and Saccharomyces cerevisiae, amplification in Escherichia coli Candida albicans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Saccharomyces cerevisiae
-
 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Candida albicans
-
 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Cryptococcus neoformans
-
 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+ Aspergillus fumigatus
-
 2-oxoadipate + CO2 + NADH
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus
-
-
-
Candida albicans
-
-
-
Cryptococcus neoformans
-
-
-
Saccharomyces cerevisiae
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 mutant 18804 1.4 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein Candida albicans
additional information
-
 1.2 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein Saccharomyces cerevisiae
additional information
-
 several mutant with about 0.1 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein Cryptococcus neoformans
additional information
-
 0.1 SE, 1 SE is change in absorption at wavelength 520 nm per 2 h per milligram of protein Aspergillus fumigatus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Saccharomyces cerevisiae 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Candida albicans 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Cryptococcus neoformans 2-oxoadipate + CO2 + NADH
-
 ?
1-hydroxy-1,2,4-butanetricarboxylate + NAD+
-
 Aspergillus fumigatus 2-oxoadipate + CO2 + NADH
-
 ?