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Literature summary for 1.1.1.86 extracted from

  • Omura, F.
    Targeting of mitochondrial Saccharomyces cerevisiae Ilv5p to the cytosol and its effect on vicinal diketone formation in brewing (2008), Appl. Microbiol. Biotechnol., 78, 503-513.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressd in Escherichia coli and Saccharomyces cerevisiae Saccharomyces cerevisiae

Protein Variants

Protein Variants Comment Organism
DELTA1-17 mutant with N-terminal 17-residue deletion: cellular localisation similar to wild-type, introduction into an ilv5DELTA strain, lacking the ilv5 gene, complements isoleucine and valine synthesis Saccharomyces cerevisiae
DELTA1-33 mutant with N-terminal 33-residue deletion: cellular localisation predominatly in the cytosol rather than in mitochondria. Deletion of the N-terminal 33 residues is sufficient to largely impair the protein's targeting to mitochondria. Introduction into an ilv5DELTA strain, lacking the ilv5 gene, complements isoleucine and valine synthesis Saccharomyces cerevisiae
DELTA1-40 mutant with N-terminal 40-residue deletion: cellular localisation throughout the cytosol rather than in mitochondria Saccharomyces cerevisiae
DELTA1-46 mutant with N-terminal 46-residue deletion: cellular localisation throughout the cytosol rather than in mitochondria. This mutant shows the largest steady-state protein level. Introduction into an ilv5DELTA strain, lacking the ilv5 gene, does not complements isoleucine and valine synthesis. Introduction into an industrial lager brewing strain, a robust expression of DELTA46 is as effective as that of a wild-type Ilv5p in lowering the total Vicinal diketones production in a 2l scale fermentation trial. Additional expression of DELTA46 does not alter the quality of the resultant beer in terms of contents of aromatic compounds and organic acids Saccharomyces cerevisiae
DELTA1-53 mutant with N-terminal 53-residue deletion: cellular localisation throughout the cytosol rather than in mitochondria Saccharomyces cerevisiae
DELTA1-66 mutant with N-terminal 66-residue deletion: weaker cytosolic localisation compared to other mutants. Mutant shows only a small amount of steady-state protein content Saccharomyces cerevisiae
DELTA1-83 mutant with N-terminal 83-residue deletion: weaker cytosolic localisation compared to other mutants. Mutant shows only a small amount of steady-state protein content Saccharomyces cerevisiae
DELTA1-99 mutant with N-terminal 99-residue deletion: weaker cytosolic localisation compared to other mutants. Mutant shows only a small amount of steady-state protein content Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
mitochondrion
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 Saccharomyces cerevisiae 5739
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Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
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-
-

Synonyms

Synonyms Comment Organism
Ilv5p
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 Saccharomyces cerevisiae
ketol-acid reductoisomerase
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 Saccharomyces cerevisiae