Cloned (Comment) | Organism |
---|---|
expression as His6-tagged enzyme | Escherichia coli |
Crystallization (Comment) | Organism |
---|---|
purified recombinant His-tagged enzyme, 9 mg/ml protein in 20 mM sodium HEPES, pH 7.5, and NADPH in a ratio of 10 mol NADPH per mol of enzyme, hanging drop vapour diffusion method, equal volumes of 0.003 ml of protein and reservoir solution, the latter containing 1.6 M ammonium sulfate, and 0.1 M sodium bicine, pH 9.0, 17°C, 6 months, X-ray diffraction structure determination and analysis at 2.6 A resolution | Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+ | active site structure | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+ | the enzyme is involved in biosynthesis of the branched chain amino acids valine and leucine, pathway overview | Escherichia coli | (S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | three-dimensional enzyme structure, subunit domain structures, surface contact and interlock, crystal structure analysis, overview | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
class II ketol-acid reductoisomerase | - |
Escherichia coli |
KARI | - |
Escherichia coli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Escherichia coli |