Literature summary for 1.1.1.86 extracted from

Tyagi, R.; Duquerroy, S.; Navaza, J.; Guddat, L.W.; Duggleby, R.G.
The crystal structure of a bacterial class II ketol-acid reductoisomerase: domain conservation and evolution (2005), Protein Sci., 14, 3089-3100.

Cloned(Commentary)

Cloned (Comment)	Organism
expression as His6-tagged enzyme	Escherichia coli

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged enzyme, 9 mg/ml protein in 20 mM sodium HEPES, pH 7.5, and NADPH in a ratio of 10 mol NADPH per mol of enzyme, hanging drop vapour diffusion method, equal volumes of 0.003 ml of protein and reservoir solution, the latter containing 1.6 M ammonium sulfate, and 0.1 M sodium bicine, pH 9.0, 17°C, 6 months, X-ray diffraction structure determination and analysis at 2.6 A resolution	Escherichia coli

Crystallization (Comment)

Organism

purified recombinant His-tagged enzyme, 9 mg/ml protein in 20 mM sodium HEPES, pH 7.5, and NADPH in a ratio of 10 mol NADPH per mol of enzyme, hanging drop vapour diffusion method, equal volumes of 0.003 ml of protein and reservoir solution, the latter containing 1.6 M ammonium sulfate, and 0.1 M sodium bicine, pH 9.0, 17°C, 6 months, X-ray diffraction structure determination and analysis at 2.6 A resolution

Escherichia coli

Organism

Organism	UniProt	Comment	Textmining
Escherichia coli	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant His6-tagged enzyme	Escherichia coli

Reaction

Reaction	Comment	Organism	Reaction ID
(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH + H+	active site structure	Escherichia coli	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-2,3-dihydroxy-3-methylbutanoate + NADP+	the enzyme is involved in biosynthesis of the branched chain amino acids valine and leucine, pathway overview	Escherichia coli	(S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH	-	?

Subunits

Subunits	Comment	Organism
tetramer	three-dimensional enzyme structure, subunit domain structures, surface contact and interlock, crystal structure analysis, overview	Escherichia coli

Synonyms

Synonyms	Comment	Organism
class II ketol-acid reductoisomerase	-	Escherichia coli
KARI	-	Escherichia coli

Cofactor

Cofactor	Comment	Organism	Structure
NADP+	-	Escherichia coli