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Literature summary for 1.1.1.86 extracted from
- A loop deletion in the plant acetohydroxy acid isomeroreductase homodimer generates an active monomer with reduced stability and altered magnesium affinity (1998), Biochemistry, 37, 12753-12760.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| mutant enzyme DELTA423-431/F431S, expression in Escherichia coli | Spinacia oleracea |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| DELTA423-430/F431S | mutant enzyme behaves as an active monomer with reduced thermal stability, KM-value for NADPH does not differ considerably from that for the wild-type enzyme, magnesium affinity is dramatically altered by monomerization | Spinacia oleracea |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| N-Hydroxy-N-isopropyloxamate | - |
Spinacia oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0035 | - |
NADPH | pH 8.2, 30°C, wild type enzyme | Spinacia oleracea | |
| 0.0052 | - |
NADPH | pH 8.2, 30°C, deletion mutant DELTA423-430/F431S | Spinacia oleracea | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 60000 | - |
monomeric mutant enzyme DELTA423-431/F431S, gel filtration | Spinacia oleracea |
| 110000 | - |
dimeric wild-type enzyme, gel filtration | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | Spinacia oleracea | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant enzyme DELTA423-431/F431S | Spinacia oleracea |
| recombinant enzyme from Escherichia coli | Spinacia oleracea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3 | - |
deletion mutant DELTA423-430/F431S | Spinacia oleracea |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -80°, 20 mM MOPS, 10% glycerol, several months, mutant enzyme DELTA423-431/F431S, no loss of activity | Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Spinacia oleracea | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| NADPH + 2-acetolactate | - |
Spinacia oleracea | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
deletion mutant DELTA423-4301/F431S shows significantly lower temperature stability than wild type enzyme | Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Spinacia oleracea | |
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Release 2023.1 (January 2023)
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