Any feedback?
Literature summary for 1.1.1.86 extracted from
- Purification and characterization of acetohydroxyacid reductoisomerase from spinach chloroplasts (1989), Biochem. J., 262, 971-976.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | competitive with NADPH | Spinacia oleracea |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0057 | - |
NADPH | pH 8, 30°C, isoenzyme 1 | Spinacia oleracea | |
| 0.025 | - |
2-acetolactate | pH 8, 30°C, isoenzyme 1 | Spinacia oleracea | |
| 0.037 | - |
2-aceto-2-hydroxybutyrate | pH 8, 30°C, isoenzyme 1 | Spinacia oleracea | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Spinacia oleracea | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | required | Spinacia oleracea | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 59000 | - |
4 * 59000, SDS-PAGE | Spinacia oleracea |
| 205000 | - |
SDS-PAGE, isoenzyme 3 | Spinacia oleracea |
| 220000 | - |
SDS-PAGE, isoenzyme 2 | Spinacia oleracea |
| 235000 | - |
SDS-PAGE, isoenzyme 1 | Spinacia oleracea |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | Spinacia oleracea | enzyme of branched chain amino acid synthesis | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Spinacia oleracea | - |
spinach, multiple enzyme forms: iso 1, 2 and 3 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Spinacia oleracea |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3.79 | - |
- |
Spinacia oleracea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2-acetolactate + NADPH | enzyme of branched chain amino acid synthesis | Spinacia oleracea | 2,3-dihydroxy-3-methylbutanoate + NADP+ | - |
r | |
| NADPH + 2-aceto-2-hydroxybutyrate | - |
Spinacia oleracea | NADP+ + ? | - |
? | |
| NADPH + 2-acetolactate | - |
Spinacia oleracea | NADP+ + 3-hydroxy-3-methyl-2-oxobutyrate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| tetramer | 4 * 59000, SDS-PAGE | Spinacia oleracea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | 8 | reaction with 2-acetolactate, isoenzyme 1 | Spinacia oleracea |
| 8 | 8.5 | reaction with 2-aceto-2-hydroxybutyrate | Spinacia oleracea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | 8.5 | pH 6.5: about 35% of maximal activity, pH 8.5: about 90% of maximal activity, reaction with 2-acetolactate, isoenzyme 1 | Spinacia oleracea |
| 7 | 8.5 | pH 7.0: about 25% of maximal activity, pH 8.0-8.5: optimum, isoenzyme 1 | Spinacia oleracea |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADPH | - |
Spinacia oleracea | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
