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Literature summary for 1.1.1.85 extracted from

  • Kotsuka, T.; Akanuma, S.; Tomuro, M.; Yamagishi, A.; Oshima, T.
    Further stabilization of 3-isopropylmalate dehydrogenase of an extreme thermophile, Thermus thermophilus, by a suppressor mutation method (1996), J. Bacteriol., 178, 723-727 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

Protein Variants Comment Organism
A172V mutation improves thermal stability, without significantly changing its catalytic properties Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.022
-
(2R,3S)-3-isopropylmalate 60°C, pH 7.6, mutant enzyme A172V Thermus thermophilus
0.031
-
(2R,3S)-3-isopropylmalate 60°C, pH 7.6, wild-type enzyme Thermus thermophilus
0.13
-
NAD+ 60°C, pH 7.6, pH 7.6, mutant enzyme A172V Thermus thermophilus
0.14
-
NAD+ 60°C, pH 7.6, wild-type enzyme Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus P61494
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermus thermophilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
Thermus thermophilus (2S)-2-isopropyl-3-oxosuccinate + NADH + H+
-
?

Subunits

Subunits Comment Organism
dimer
-
Thermus thermophilus

Synonyms

Synonyms Comment Organism
IPMDH
-
Thermus thermophilus
leuB
-
Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
163
-
NAD+ 60°C, pH 7.6, wild-type enzyme Thermus thermophilus
163
-
(2R,3S)-3-isopropylmalate 60°C, pH 7.6, wild-type enzyme Thermus thermophilus
220
-
(2R,3S)-3-isopropylmalate 60°C, pH 7.6, mutant enzyme A172V Thermus thermophilus
220
-
NAD+ 60°C, pH 7.6, pH 7.6, mutant enzyme A172V Thermus thermophilus