Protein Variants | Comment | Organism |
---|---|---|
A172V | mutation improves thermal stability, without significantly changing its catalytic properties | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
(2R,3S)-3-isopropylmalate | 60°C, pH 7.6, mutant enzyme A172V | Thermus thermophilus | |
0.031 | - |
(2R,3S)-3-isopropylmalate | 60°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
0.13 | - |
NAD+ | 60°C, pH 7.6, pH 7.6, mutant enzyme A172V | Thermus thermophilus | |
0.14 | - |
NAD+ | 60°C, pH 7.6, wild-type enzyme | Thermus thermophilus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | P61494 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-3-isopropylmalate + NAD+ | - |
Thermus thermophilus | (2S)-2-isopropyl-3-oxosuccinate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | - |
Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
IPMDH | - |
Thermus thermophilus |
leuB | - |
Thermus thermophilus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
163 | - |
NAD+ | 60°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
163 | - |
(2R,3S)-3-isopropylmalate | 60°C, pH 7.6, wild-type enzyme | Thermus thermophilus | |
220 | - |
(2R,3S)-3-isopropylmalate | 60°C, pH 7.6, mutant enzyme A172V | Thermus thermophilus | |
220 | - |
NAD+ | 60°C, pH 7.6, pH 7.6, mutant enzyme A172V | Thermus thermophilus |