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Literature summary for 1.1.1.85 extracted from

  • Suzuki, T.; Yasugi, M.; Arisaka, F.; Oshima, T.; Yamagishi, A.
    Cold-adaptation mechanism of mutant enzymes of 3-isopropylmalate dehydrogenase from Thermus thermophilus (2002), Protein Eng., 15, 471-476.
    View publication on PubMed
Search for more literature for 1.1.1.85

Protein Variants

Protein Variants Comment Organism
V126A the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
V126E the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
V126F the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
V126G the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
V126I the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
V126L the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
V126M the mutation increases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
V126S the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus
V126T the mutation decreases the KM value of DL-3-isopropylmalate at 60°C in comparison to wild-type Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.002
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126G Thermus thermophilus
0.0026
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126A Thermus thermophilus
0.0027
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126T Thermus thermophilus
0.0032
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126S Thermus thermophilus
0.0044
-
 DL-3-isopropylmalate pH 8.0, 60°C, wild-type Thermus thermophilus
0.005
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126M Thermus thermophilus
0.0061
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126E Thermus thermophilus
0.0084
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126I Thermus thermophilus
0.0091
-
 NAD+ pH 8.0, 40°C, mutant V126S Thermus thermophilus
0.0092
-
 NAD+ pH 8.0, 40°C, mutant V126A Thermus thermophilus
0.0121
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126L Thermus thermophilus
0.0133
-
 DL-3-isopropylmalate pH 8.0, 60°C, mutant V126F Thermus thermophilus
0.022
-
 NAD+ pH 8.0, 40°C, mutant V126T Thermus thermophilus
0.024
-
 NAD+ pH 8.0, 40°C, mutant V126G Thermus thermophilus
0.025
-
 NAD+ pH 8.0, 40°C, wild-type Thermus thermophilus
0.0515
-
 NAD+ pH 8.0, 40°C, mutant V126E Thermus thermophilus
0.059
-
 NAD+ pH 8.0, 60°C, mutant V126A Thermus thermophilus
0.062
-
 NAD+ pH 8.0, 40°C, mutant V126I Thermus thermophilus
0.0725
-
 NAD+ pH 8.0, 60°C, mutant V126G Thermus thermophilus
0.095
-
 NAD+ pH 8.0, 60°C, mutant V126S Thermus thermophilus
0.103
-
 NAD+ pH 8.0, 60°C, mutant V126T Thermus thermophilus
0.109
-
 NAD+ pH 8.0, 60°C, mutant V126E Thermus thermophilus
0.111
-
 NAD+ pH 8.0, 40°C, mutant V126L Thermus thermophilus
0.144
-
 NAD+ pH 8.0, 40°C, mutant V126F Thermus thermophilus
0.166
-
 NAD+ pH 8.0, 60°C, wild-type Thermus thermophilus
0.198
-
 NAD+ pH 8.0, 60°C, mutant V126I Thermus thermophilus
0.378
-
 NAD+ pH 8.0, 60°C, mutant V126F Thermus thermophilus
0.391
-
 NAD+ pH 8.0, 60°C, mutant V126L Thermus thermophilus
1.01
-
 NAD+ pH 8.0, 40°C, mutant V126M Thermus thermophilus
1.97
-
 NAD+ pH 8.0, 60°C, mutant V126M Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
DL-3-isopropylmalate + NAD+
-
 Thermus thermophilus DL-isopropyl-3-oxosuccinate + NADH + H+
-
 ?

Synonyms

Synonyms Comment Organism
3-isopropylmalate dehydrogenase
-
 Thermus thermophilus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
additional information
-
 additional information the cold adaption results from the destabilization of the ternary complex caused by increase in the volume of the residue at position 126 Thermus thermophilus
1.2
-
 NAD+ pH 8.0, 40°C, mutant V126A Thermus thermophilus
1.5
-
 NAD+ pH 8.0, 40°C, mutant V126S Thermus thermophilus
1.7
-
 NAD+ pH 8.0, 40°C, mutant V126G Thermus thermophilus
1.9
-
 NAD+ pH 8.0, 40°C, mutant V126T Thermus thermophilus
2.4
-
 NAD+ pH 8.0, 40°C, wild-type Thermus thermophilus
3.5
-
 NAD+ pH 8.0, 40°C, mutant V126E Thermus thermophilus
6
-
 NAD+ pH 8.0, 40°C, mutant V126I Thermus thermophilus
8.5
-
 NAD+ pH 8.0, 40°C, mutant V126L Thermus thermophilus
9
-
 NAD+ pH 8.0, 60°C, mutant V126A Thermus thermophilus
10
-
 NAD+ pH 8.0, 60°C, V126G Thermus thermophilus
11
-
 NAD+ pH 8.0, 40°C, mutant V126F Thermus thermophilus
13
-
 NAD+ pH 8.0, 60°C, mutant V126S Thermus thermophilus
15
-
 NAD+ pH 8.0, 60°C, mutant V126T Thermus thermophilus
18
-
 NAD+ pH 8.0, 40°C, mutant V126M Thermus thermophilus
20
-
 NAD+ pH 8.0, 60°C, wild-type Thermus thermophilus
21
-
 NAD+ pH 8.0, 60°C, mutant V126E Thermus thermophilus
30.5
-
 NAD+ pH 8.0, 60°C, mutant V126I Thermus thermophilus
42
-
 NAD+ pH 8.0, 60°C, mutant V126F Thermus thermophilus
44.5
-
 NAD+ pH 8.0, 60°C, mutant V126L Thermus thermophilus
54
-
 NAD+ pH 8.0, 60°C, mutant V126M Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermus thermophilus