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Literature summary for 1.1.1.85 extracted from

  • Chen, R.; Greer, A.; Dean, A.M.
    Redesigning secondary structure to invert coenzyme specificity in isopropylmalate dehydrogenase (1996), Proc. Natl. Acad. Sci. USA, 93, 12171-12176.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
S226R Km-value for NADP+ is about 40% compared to wild-type enzyme, Km-value for NAD+ is 2.6fold higher compared to wild-type enzyme Thermus thermophilus
S226R/D278K/I279Y Km-value for NADP+ is about 0.8% compared to wild-type enzyme, Km-value for NAD+ is 153fold higher compared to wild-type enzyme Thermus thermophilus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
 NAD+ pH 7.3, 21°C, wild-type enzyme Thermus thermophilus
0.014
-
 NADP+ pH 7.3, 21°C, mutant enzyme S226R/D278K/I279Y Thermus thermophilus
0.02
-
 NADP+ pH 7.3, 21°C, mutant enzyme D278K, I279T, P325Y and 395-Arg Thermus thermophilus
0.031
-
 NAD+ pH 7.3, 21°C, mutant enzyme S226R Thermus thermophilus
0.722
-
 NADP+ pH 7.3, 21°C, mutant enzyme S226R Thermus thermophilus
1.75
-
 NADP+ wild-type enzyme Thermus thermophilus
1.836
-
 NAD+ pH 7.3, 21°C, mutant enzyme S226R/D278K/I279Y Thermus thermophilus

Organism

Organism UniProt Comment Textmining
Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3S)-3-isopropylmalate + NAD+
-
 Thermus thermophilus 2-oxoisocaproate + NADH + H+ + CO2
-
 ?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.09
-
 isopropylmalate pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R/D278K/I279Y Thermus thermophilus
0.09
-
 NADP+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y Thermus thermophilus
0.15
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, wild-type enzyme Thermus thermophilus
0.15
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
0.26
-
 isopropylmalate pH 7.3, 21°C, reaction with NADP+, wild-type enzyme Thermus thermophilus
0.26
-
 NADP+ pH 7.3, 21°C, reaction with isopropylmalate, wild-type enzyme Thermus thermophilus
0.48
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R Thermus thermophilus
0.48
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R Thermus thermophilus
0.88
-
 NADP+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R Thermus thermophilus
0.88
-
 isopropylmalate pH 7.3, 21°C, reaction with NADP+, mutant enzyme S226R Thermus thermophilus
1.91
-
 NAD+ pH 7.3, 21°C, reaction with isopropylmalate, mutant enzyme S226R/D278K/I279Y Thermus thermophilus
1.91
-
 isopropylmalate pH 7.3, 21°C, reaction with NAD+, mutant enzyme S226R/D278K/I279Y Thermus thermophilus

Cofactor

Cofactor Comment Organism Structure
NAD+
-
 Thermus thermophilus