Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | thioredoxin-dependent reduction steps are involved in NADP-dependent malate dehydrogenase light activation | Sorghum sp. | |
thioredoxin | thioredoxin-dependent reduction steps are involved in NADP-dependent malate dehydrogenase light activation | Sorghum sp. |
Cloned (Comment) | Organism |
---|---|
high-level expression in Escherichia coli, wild-type enzyme and mutant enzymes C64S, C69S, C64S/C69S and DELTAN, a truncation mutant corresponding to the deletion of the 5' end of the mdh cDNA open reading frame until the 73rd codon | Sorghum sp. |
Protein Variants | Comment | Organism |
---|---|---|
C64S | still requires activation by reduced thioredoxin, activation is almost instantaneous, whereas the native enzyme reaches full activity after 10-20 min of preincubation, the half-saturation concentration for reduced thioredoxin is decreased 2fold | Sorghum sp. |
C64S/C69S | still requires activation by reduced thioredoxin, activation is almost instantaneous, whereas the native enzyme reaches full activity after 10-20 min of preincubation, the half-saturation concentration for reduced thioredoxin is decreased 2fold | Sorghum sp. |
C69S | still requires activation by reduced thioredoxin, activation is almost instantaneous, whereas the native enzyme reaches full activity after 10-20 min of preincubation, the half-saturation concentration for reduced thioredoxin is decreased 2fold | Sorghum sp. |
DELTAN | truncation mutant corresponding to the deletion of the 5' end of the mdh cDNA open reading frame until the 73rd codon | Sorghum sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.022 | - |
oxaloacetate | mutant enzyme DELTAN | Sorghum sp. | |
0.028 | - |
NADPH | mutant enzyme C64S/C69S | Sorghum sp. | |
0.037 | - |
oxaloacetate | mutant enzyme C64S | Sorghum sp. | |
0.037 | - |
NADPH | mutant C69S | Sorghum sp. | |
0.038 | - |
NADPH | mutant enzyme C64S | Sorghum sp. | |
0.039 | - |
NADPH | mutant enzyme DELTAN | Sorghum sp. | |
0.041 | - |
oxaloacetate | wild-type enzyme | Sorghum sp. | |
0.042 | - |
NADPH | wild-type enzyme | Sorghum sp. | |
0.042 | - |
oxaloacetate | mutant C69S | Sorghum sp. | |
0.044 | - |
oxaloacetate | mutant enzyme C64S/C69S | Sorghum sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Sorghum sp. | 9507 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 33000, DELTAN, a truncation mutant corresponding to the deletion of the 5 end of the mdh cDNA open reading frame until the 73rd codon, SDS-PAGE | Sorghum sp. |
40000 | - |
x * 40000, wild-type enzyme and mutant enzymes C64S, C69S, C64S/C69S, SDS-PAGE | Sorghum sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Sorghum sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Sorghum sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Sorghum sp. | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADPH | - |
Sorghum sp. | (S)-malate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, wild-type enzyme and mutant enzymes C64S, C69S, C64S/C69S, SDS-PAGE | Sorghum sp. |
? | x * 33000, DELTAN, a truncation mutant corresponding to the deletion of the 5' end of the mdh cDNA open reading frame until the 73rd codon, SDS-PAGE | Sorghum sp. |