KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0145 | - |
(S)-malate | - |
Pisum sativum | |
0.039 | - |
NADPH | - |
Pisum sativum | |
0.048 | - |
oxaloacetate | - |
Pisum sativum | |
0.063 | - |
NADP+ | - |
Pisum sativum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | not essential, stimulates up to 20%, optimal concentration is 10 mM MgCl2 | Pisum sativum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38500 | - |
1 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Pisum sativum |
38500 | - |
2 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Pisum sativum |
38500 | - |
4 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Pisum sativum |
40000 | - |
monomer, gel filtration | Pisum sativum |
87000 | - |
dimer, gel filtration | Pisum sativum |
163000 | - |
tetramer, gel filtration | Pisum sativum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pisum sativum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Pisum sativum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Pisum sativum | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
184 | - |
- |
Pisum sativum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Pisum sativum | oxaloacetate + NADPH + H+ | - |
r | |
oxaloacetate + NADPH | - |
Pisum sativum | (S)-malate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Pisum sativum |
monomer | 1 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Pisum sativum |
More | enzyme exists as monomer dimer and tetramer. At neutral pH, pH 7.0-8.6, the enzyme is tetrameric | Pisum sativum |
tetramer | 4 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE | Pisum sativum |