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Literature summary for 1.1.1.82 extracted from

  • Fickenscher, K.; Scheibe, R.
    Purification and properties of NADP-dependent malate dehydrogenase from pea leaves (1983), Biochim. Biophys. Acta, 749, 249-254.
No PubMed abstract available

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0145
-
(S)-malate
-
Pisum sativum
0.039
-
NADPH
-
Pisum sativum
0.048
-
oxaloacetate
-
Pisum sativum
0.063
-
NADP+
-
Pisum sativum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ not essential, stimulates up to 20%, optimal concentration is 10 mM MgCl2 Pisum sativum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
38500
-
1 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
38500
-
2 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
38500
-
4 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
40000
-
monomer, gel filtration Pisum sativum
87000
-
dimer, gel filtration Pisum sativum
163000
-
tetramer, gel filtration Pisum sativum

Organism

Organism UniProt Comment Textmining
Pisum sativum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Pisum sativum

Source Tissue

Source Tissue Comment Organism Textmining
leaf
-
Pisum sativum
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
184
-
-
Pisum sativum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NADP+
-
Pisum sativum oxaloacetate + NADPH + H+
-
r
oxaloacetate + NADPH
-
Pisum sativum (S)-malate + NADP+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
monomer 1 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum
More enzyme exists as monomer dimer and tetramer. At neutral pH, pH 7.0-8.6, the enzyme is tetrameric Pisum sativum
tetramer 4 * 38500, enzyme exists as monomer, dimer and tetramer, SDS-PAGE Pisum sativum