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Literature summary for 1.1.1.81 extracted from

  • But, S.Y.; Egorova, S.V.; Khmelenina, V.N.; Trotsenko, Y.A.
    Biochemical properties and phylogeny of hydroxypyruvate reductases from methanotrophic bacteria with different C1-assimilation pathways (2017), Biochemistry (Moscow), 82, 1295-1303 .
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
2-oxoglutarate 5 mM, 231% of initial activity Methylotuvimicrobium alcaliphilum
acetyl-CoA 5 mM, 120% of initial activity Methylosinus trichosporium
ADP 5 mM, 298% of initial activity Methylotuvimicrobium alcaliphilum
ATP 5 mM, 305% of initial activity Methylotuvimicrobium alcaliphilum
citrate 5 mM, 399% of initial activity Methylotuvimicrobium alcaliphilum
diphosphate 5 mM, 152% of initial activity Methylosinus trichosporium
diphosphate 5 mM, 362% of initial activity Methylotuvimicrobium alcaliphilum
fructose 1,6-diphosphate 5 mM, 244% of initial activity Methylotuvimicrobium alcaliphilum
glucose 6-phosphate 5 mM, 134% of initial activity Methylosinus trichosporium
glucose 6-phosphate 5 mM, 163% of initial activity Methylotuvimicrobium alcaliphilum
glycerate 5 mM, 244% of initial activity Methylococcus capsulatus
NAD+ 1 mM, substrate NADH, 157% of initial activity Methylotuvimicrobium alcaliphilum
NADP+ 1 mM, substrate NADH, 104% of initial activity Methylococcus capsulatus
NADP+ 1 mM, substrate NADH, 114% of initial activity Methylotuvimicrobium alcaliphilum
NADP+ 1 mM, substrate NADPH, 199% of initial activity Methylotuvimicrobium alcaliphilum
NADP+ 1 mM, substrate NADPH, 200% of initial activity Methylococcus capsulatus
oxaloacetate 5 mM, 119% of initial activity Methylosinus trichosporium
oxaloacetate 5 mM, 231% of initial activity Methylotuvimicrobium alcaliphilum
Ribulose 1,5-diphosphate 5 mM, 170% of initial activity Methylosinus trichosporium
Ribulose 1,5-diphosphate 5 mM, 325% of initial activity Methylotuvimicrobium alcaliphilum
ribulose 5-phosphate 5 mM, 109% of initial activity Methylosinus trichosporium
serine 5 mM, 180% of initial activity Methylotuvimicrobium alcaliphilum

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Methylosinus trichosporium
expression in Escherichia coli Methylotuvimicrobium alcaliphilum
expression in Escherichia coli Methylococcus capsulatus

Inhibitors

Inhibitors Comment Organism Structure
2-oxoglutarate 5 mM, 80% of initial activity Methylosinus trichosporium
acetyl-CoA 5 mM, 58% of initial activity Methylococcus capsulatus
acetyl-CoA 5 mM, 58% of initial activity Methylotuvimicrobium alcaliphilum
ADP 5 mM, 94% of initial activity Methylococcus capsulatus
ADP 5 mM, 78% of initial activity Methylosinus trichosporium
ATP 5 mM, 78% of initial activity Methylococcus capsulatus
ATP 5 mM, 57% of initial activity Methylosinus trichosporium
glucose 6-phosphate 5 mM, 49% of initial activity Methylococcus capsulatus
glucose 6-phosphate 5 mM, 91% of initial activity Methylosinus trichosporium
NAD+ 1 mM, substrate NADH, 92% of initial activity; 1 mM, substrate NADPH, 54% of initial activity Methylococcus capsulatus
NAD+ 1 mM, substrate NADH, 86% of initial activity; 1 mM, substrate NADPH, 72% of initial activity Methylosinus trichosporium
NAD+ 1 mM, substrate NADPH, 64% of initial activity Methylotuvimicrobium alcaliphilum
NADP+ 1 mM, substrate NADH, 81% of initial activity; 1 mM, substrate NADPH, 87% of initial activity Methylosinus trichosporium
phosphate 5 mM, 67% of initial activity Methylococcus capsulatus
phosphate 5 mM, 67% of initial activity Methylotuvimicrobium alcaliphilum
phosphoenolpyruvate 5 mM, 41% of initial activity Methylococcus capsulatus
phosphoenolpyruvate 5 mM, 41% of initial activity Methylosinus trichosporium
phosphoenolpyruvate 5 mM, 41% of initial activity Methylotuvimicrobium alcaliphilum
pyruvate 5 mM, 78% of initial activity Methylococcus capsulatus
pyruvate 5 mM, 67% of initial activity Methylosinus trichosporium
pyruvate 5 mM, 78% of initial activity Methylotuvimicrobium alcaliphilum
ribulose 5-phosphate 5 mM, 47% of initial activity Methylococcus capsulatus
ribulose 5-phosphate 5 mM, 47% of initial activity Methylotuvimicrobium alcaliphilum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.013
-
NADH cosubsubstrate glyoxylate, 30°C, pH 7 Methylosinus trichosporium
0.015
-
NADH cosubsubstrate glyoxylate, 37°C, pH 7 Methylococcus capsulatus
0.028
-
NADPH cosubsubstrate glyoxylate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
0.028
-
NADH cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 Methylosinus trichosporium
0.03
-
NADH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylococcus capsulatus
0.055
-
NADPH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
0.057
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 37°C Methylococcus capsulatus
0.07
-
NADH cosubsubstrate glyoxylate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
0.08
-
NADH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
0.11
-
NADPH cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 Methylosinus trichosporium
0.14
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 30°C Methylosinus trichosporium
0.16
-
NADPH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylococcus capsulatus
0.17
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
0.22
-
NADPH cosubsubstrate glyoxylate, 30°C, pH 7 Methylosinus trichosporium
0.5
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 37°C Methylococcus capsulatus
0.6
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
1.2
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 30°C Methylosinus trichosporium
3
-
glyoxylate cosubstrate NADH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
3.9
-
glyoxylate cosubstrate NADH, pH 7, 37°C Methylococcus capsulatus
11
-
glyoxylate cosubstrate NADPH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
18
-
glyoxylate cosubstrate NADPH, pH 7, 30°C Methylosinus trichosporium
22
-
glyoxylate cosubstrate NADH, pH 7, 30°C Methylosinus trichosporium

Organism

Organism UniProt Comment Textmining
Methylococcus capsulatus Q608T2
-
-
Methylococcus capsulatus Bath Q608T2
-
-
Methylosinus trichosporium
-
-
-
Methylotuvimicrobium alcaliphilum
-
-
-
Methylotuvimicrobium alcaliphilum 20Z
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-hydroxypyruvate + NADH + H+
-
Methylosinus trichosporium D-glycerate + NAD+
-
ir
3-hydroxypyruvate + NADH + H+
-
Methylotuvimicrobium alcaliphilum D-glycerate + NAD+
-
ir
3-hydroxypyruvate + NADH + H+
-
Methylococcus capsulatus D-glycerate + NAD+
-
ir
3-hydroxypyruvate + NADH + H+
-
Methylococcus capsulatus Bath D-glycerate + NAD+
-
ir
3-hydroxypyruvate + NADH + H+
-
Methylotuvimicrobium alcaliphilum 20Z D-glycerate + NAD+
-
ir
3-hydroxypyruvate + NADPH + H+
-
Methylosinus trichosporium D-glycerate + NADP+
-
ir
3-hydroxypyruvate + NADPH + H+
-
Methylotuvimicrobium alcaliphilum D-glycerate + NADP+
-
ir
3-hydroxypyruvate + NADPH + H+
-
Methylococcus capsulatus D-glycerate + NADP+
-
ir
3-hydroxypyruvate + NADPH + H+
-
Methylococcus capsulatus Bath D-glycerate + NADP+
-
ir
3-hydroxypyruvate + NADPH + H+
-
Methylotuvimicrobium alcaliphilum 20Z D-glycerate + NADP+
-
ir
glyoxylate + NADH + H+ 24% of the activity with 3-hydroxypyruvate Methylosinus trichosporium glycolate + NAD+
-
ir
glyoxylate + NADH + H+ 60% of the activity with 3-hydroxypyruvate Methylotuvimicrobium alcaliphilum glycolate + NAD+
-
ir
glyoxylate + NADH + H+ 97% of the activity with 3-hydroxypyruvate Methylococcus capsulatus glycolate + NAD+
-
ir
glyoxylate + NADH + H+ 97% of the activity with 3-hydroxypyruvate Methylococcus capsulatus Bath glycolate + NAD+
-
ir
glyoxylate + NADH + H+ 60% of the activity with 3-hydroxypyruvate Methylotuvimicrobium alcaliphilum 20Z glycolate + NAD+
-
ir
additional information enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate Methylosinus trichosporium ?
-
-
additional information enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate Methylotuvimicrobium alcaliphilum ?
-
-
additional information enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate Methylococcus capsulatus ?
-
-
additional information enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate Methylococcus capsulatus Bath ?
-
-
additional information enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate Methylotuvimicrobium alcaliphilum 20Z ?
-
-

Synonyms

Synonyms Comment Organism
DVE02_v2_12566
-
Methylosinus trichosporium
HPR
-
Methylosinus trichosporium
HPR
-
Methylotuvimicrobium alcaliphilum
HPR
-
Methylococcus capsulatus
LCv4_3219
-
Methylotuvimicrobium alcaliphilum
MCA1407
-
Methylococcus capsulatus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
Methylosinus trichosporium
60
-
-
Methylotuvimicrobium alcaliphilum
60
-
-
Methylococcus capsulatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
14
-
NADH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
19
-
glyoxylate cosubstrate NADH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
19
-
NADH cosubsubstrate glyoxylate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
23
-
NADPH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylococcus capsulatus
30
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
38
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 37°C Methylococcus capsulatus
41
-
glyoxylate cosubstrate NADH, pH 7, 37°C Methylococcus capsulatus
41
-
NADH cosubsubstrate glyoxylate, 37°C, pH 7 Methylococcus capsulatus
42
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 37°C Methylococcus capsulatus
42
-
NADH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylococcus capsulatus
48
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
48
-
NADPH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
52
-
glyoxylate cosubstrate NADPH, pH 7, 30°C Methylosinus trichosporium
52
-
NADPH cosubsubstrate glyoxylate, 30°C, pH 7 Methylosinus trichosporium
88
-
glyoxylate cosubstrate NADPH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
88
-
NADPH cosubsubstrate glyoxylate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
166
-
glyoxylate cosubstrate NADH, pH 7, 30°C Methylosinus trichosporium
166
-
NADH cosubsubstrate glyoxylate, 30°C, pH 7 Methylosinus trichosporium
284
-
NADPH cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 Methylosinus trichosporium
288
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 30°C Methylosinus trichosporium
687
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 30°C Methylosinus trichosporium
687
-
NADH cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 Methylosinus trichosporium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
Methylosinus trichosporium
7
-
-
Methylotuvimicrobium alcaliphilum
7
-
-
Methylococcus capsulatus

Cofactor

Cofactor Comment Organism Structure
NADH NADPH is preferred over NADH Methylotuvimicrobium alcaliphilum
NADH NADH is preferred over NADPH Methylosinus trichosporium
NADH NADH is preferred over NADPH Methylococcus capsulatus
NADPH NADPH is preferred over NADH Methylotuvimicrobium alcaliphilum
NADPH NADH is preferred over NADPH Methylosinus trichosporium
NADPH NADH is preferred over NADPH Methylococcus capsulatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.9
-
glyoxylate cosubstrate NADPH, pH 7, 30°C Methylosinus trichosporium
6
-
glyoxylate cosubstrate NADH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
6
-
glyoxylate cosubstrate NADH, pH 7, 37°C Methylococcus capsulatus
7.5
-
glyoxylate cosubstrate NADH, pH 7, 30°C Methylosinus trichosporium
8
-
glyoxylate cosubstrate NADPH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
10.56
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 37°C Methylococcus capsulatus
50
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
143
-
NADPH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylococcus capsulatus
175
-
NADH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
236
-
NADPH cosubsubstrate glyoxylate, 30°C, pH 7 Methylosinus trichosporium
240
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 30°C Methylosinus trichosporium
271
-
NADH cosubsubstrate glyoxylate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
282
-
3-hydroxypyruvate cosubstrate NADPH, pH 7, 37°C Methylotuvimicrobium alcaliphilum
737
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 37°C Methylococcus capsulatus
873
-
NADPH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
1400
-
NADH cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 Methylococcus capsulatus
2582
-
NADPH cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 Methylosinus trichosporium
2733
-
NADH cosubsubstrate glyoxylate, 37°C, pH 7 Methylococcus capsulatus
3142
-
NADPH cosubsubstrate glyoxylate, 37°C, pH 7 Methylotuvimicrobium alcaliphilum
4907
-
3-hydroxypyruvate cosubstrate NADH, pH 7, 30°C Methylosinus trichosporium
12769
-
NADH cosubsubstrate glyoxylate, 30°C, pH 7 Methylosinus trichosporium
24535
-
NADH cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 Methylosinus trichosporium