Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 5 mM, 231% of initial activity | Methylotuvimicrobium alcaliphilum | |
acetyl-CoA | 5 mM, 120% of initial activity | Methylosinus trichosporium | |
ADP | 5 mM, 298% of initial activity | Methylotuvimicrobium alcaliphilum | |
ATP | 5 mM, 305% of initial activity | Methylotuvimicrobium alcaliphilum | |
citrate | 5 mM, 399% of initial activity | Methylotuvimicrobium alcaliphilum | |
diphosphate | 5 mM, 152% of initial activity | Methylosinus trichosporium | |
diphosphate | 5 mM, 362% of initial activity | Methylotuvimicrobium alcaliphilum | |
fructose 1,6-diphosphate | 5 mM, 244% of initial activity | Methylotuvimicrobium alcaliphilum | |
glucose 6-phosphate | 5 mM, 134% of initial activity | Methylosinus trichosporium | |
glucose 6-phosphate | 5 mM, 163% of initial activity | Methylotuvimicrobium alcaliphilum | |
glycerate | 5 mM, 244% of initial activity | Methylococcus capsulatus | |
NAD+ | 1 mM, substrate NADH, 157% of initial activity | Methylotuvimicrobium alcaliphilum | |
NADP+ | 1 mM, substrate NADH, 104% of initial activity | Methylococcus capsulatus | |
NADP+ | 1 mM, substrate NADH, 114% of initial activity | Methylotuvimicrobium alcaliphilum | |
NADP+ | 1 mM, substrate NADPH, 199% of initial activity | Methylotuvimicrobium alcaliphilum | |
NADP+ | 1 mM, substrate NADPH, 200% of initial activity | Methylococcus capsulatus | |
oxaloacetate | 5 mM, 119% of initial activity | Methylosinus trichosporium | |
oxaloacetate | 5 mM, 231% of initial activity | Methylotuvimicrobium alcaliphilum | |
Ribulose 1,5-diphosphate | 5 mM, 170% of initial activity | Methylosinus trichosporium | |
Ribulose 1,5-diphosphate | 5 mM, 325% of initial activity | Methylotuvimicrobium alcaliphilum | |
ribulose 5-phosphate | 5 mM, 109% of initial activity | Methylosinus trichosporium | |
serine | 5 mM, 180% of initial activity | Methylotuvimicrobium alcaliphilum |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Methylosinus trichosporium |
expression in Escherichia coli | Methylotuvimicrobium alcaliphilum |
expression in Escherichia coli | Methylococcus capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-oxoglutarate | 5 mM, 80% of initial activity | Methylosinus trichosporium | |
acetyl-CoA | 5 mM, 58% of initial activity | Methylococcus capsulatus | |
acetyl-CoA | 5 mM, 58% of initial activity | Methylotuvimicrobium alcaliphilum | |
ADP | 5 mM, 94% of initial activity | Methylococcus capsulatus | |
ADP | 5 mM, 78% of initial activity | Methylosinus trichosporium | |
ATP | 5 mM, 78% of initial activity | Methylococcus capsulatus | |
ATP | 5 mM, 57% of initial activity | Methylosinus trichosporium | |
glucose 6-phosphate | 5 mM, 49% of initial activity | Methylococcus capsulatus | |
glucose 6-phosphate | 5 mM, 91% of initial activity | Methylosinus trichosporium | |
NAD+ | 1 mM, substrate NADH, 92% of initial activity; 1 mM, substrate NADPH, 54% of initial activity | Methylococcus capsulatus | |
NAD+ | 1 mM, substrate NADH, 86% of initial activity; 1 mM, substrate NADPH, 72% of initial activity | Methylosinus trichosporium | |
NAD+ | 1 mM, substrate NADPH, 64% of initial activity | Methylotuvimicrobium alcaliphilum | |
NADP+ | 1 mM, substrate NADH, 81% of initial activity; 1 mM, substrate NADPH, 87% of initial activity | Methylosinus trichosporium | |
phosphate | 5 mM, 67% of initial activity | Methylococcus capsulatus | |
phosphate | 5 mM, 67% of initial activity | Methylotuvimicrobium alcaliphilum | |
phosphoenolpyruvate | 5 mM, 41% of initial activity | Methylococcus capsulatus | |
phosphoenolpyruvate | 5 mM, 41% of initial activity | Methylosinus trichosporium | |
phosphoenolpyruvate | 5 mM, 41% of initial activity | Methylotuvimicrobium alcaliphilum | |
pyruvate | 5 mM, 78% of initial activity | Methylococcus capsulatus | |
pyruvate | 5 mM, 67% of initial activity | Methylosinus trichosporium | |
pyruvate | 5 mM, 78% of initial activity | Methylotuvimicrobium alcaliphilum | |
ribulose 5-phosphate | 5 mM, 47% of initial activity | Methylococcus capsulatus | |
ribulose 5-phosphate | 5 mM, 47% of initial activity | Methylotuvimicrobium alcaliphilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.013 | - |
NADH | cosubsubstrate glyoxylate, 30°C, pH 7 | Methylosinus trichosporium | |
0.015 | - |
NADH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylococcus capsulatus | |
0.028 | - |
NADPH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
0.028 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 | Methylosinus trichosporium | |
0.03 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylococcus capsulatus | |
0.055 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
0.057 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 37°C | Methylococcus capsulatus | |
0.07 | - |
NADH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
0.08 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
0.11 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 | Methylosinus trichosporium | |
0.14 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 30°C | Methylosinus trichosporium | |
0.16 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylococcus capsulatus | |
0.17 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
0.22 | - |
NADPH | cosubsubstrate glyoxylate, 30°C, pH 7 | Methylosinus trichosporium | |
0.5 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 37°C | Methylococcus capsulatus | |
0.6 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
1.2 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 30°C | Methylosinus trichosporium | |
3 | - |
glyoxylate | cosubstrate NADH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
3.9 | - |
glyoxylate | cosubstrate NADH, pH 7, 37°C | Methylococcus capsulatus | |
11 | - |
glyoxylate | cosubstrate NADPH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
18 | - |
glyoxylate | cosubstrate NADPH, pH 7, 30°C | Methylosinus trichosporium | |
22 | - |
glyoxylate | cosubstrate NADH, pH 7, 30°C | Methylosinus trichosporium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | Q608T2 | - |
- |
Methylococcus capsulatus Bath | Q608T2 | - |
- |
Methylosinus trichosporium | - |
- |
- |
Methylotuvimicrobium alcaliphilum | - |
- |
- |
Methylotuvimicrobium alcaliphilum 20Z | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxypyruvate + NADH + H+ | - |
Methylosinus trichosporium | D-glycerate + NAD+ | - |
ir | |
3-hydroxypyruvate + NADH + H+ | - |
Methylotuvimicrobium alcaliphilum | D-glycerate + NAD+ | - |
ir | |
3-hydroxypyruvate + NADH + H+ | - |
Methylococcus capsulatus | D-glycerate + NAD+ | - |
ir | |
3-hydroxypyruvate + NADH + H+ | - |
Methylococcus capsulatus Bath | D-glycerate + NAD+ | - |
ir | |
3-hydroxypyruvate + NADH + H+ | - |
Methylotuvimicrobium alcaliphilum 20Z | D-glycerate + NAD+ | - |
ir | |
3-hydroxypyruvate + NADPH + H+ | - |
Methylosinus trichosporium | D-glycerate + NADP+ | - |
ir | |
3-hydroxypyruvate + NADPH + H+ | - |
Methylotuvimicrobium alcaliphilum | D-glycerate + NADP+ | - |
ir | |
3-hydroxypyruvate + NADPH + H+ | - |
Methylococcus capsulatus | D-glycerate + NADP+ | - |
ir | |
3-hydroxypyruvate + NADPH + H+ | - |
Methylococcus capsulatus Bath | D-glycerate + NADP+ | - |
ir | |
3-hydroxypyruvate + NADPH + H+ | - |
Methylotuvimicrobium alcaliphilum 20Z | D-glycerate + NADP+ | - |
ir | |
glyoxylate + NADH + H+ | 24% of the activity with 3-hydroxypyruvate | Methylosinus trichosporium | glycolate + NAD+ | - |
ir | |
glyoxylate + NADH + H+ | 60% of the activity with 3-hydroxypyruvate | Methylotuvimicrobium alcaliphilum | glycolate + NAD+ | - |
ir | |
glyoxylate + NADH + H+ | 97% of the activity with 3-hydroxypyruvate | Methylococcus capsulatus | glycolate + NAD+ | - |
ir | |
glyoxylate + NADH + H+ | 97% of the activity with 3-hydroxypyruvate | Methylococcus capsulatus Bath | glycolate + NAD+ | - |
ir | |
glyoxylate + NADH + H+ | 60% of the activity with 3-hydroxypyruvate | Methylotuvimicrobium alcaliphilum 20Z | glycolate + NAD+ | - |
ir | |
additional information | enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate | Methylosinus trichosporium | ? | - |
- |
|
additional information | enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate | Methylotuvimicrobium alcaliphilum | ? | - |
- |
|
additional information | enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate | Methylococcus capsulatus | ? | - |
- |
|
additional information | enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate | Methylococcus capsulatus Bath | ? | - |
- |
|
additional information | enzyme catalyzes NAD(P)H-dependent reduction of hydroxypyruvate or glyoxylate, but does not catalyze the reverse reactions of D-glycerate or glycolate oxidation. No substrates: pyruvate, lactate, oxaloacetate, and 2-oxoglutarate | Methylotuvimicrobium alcaliphilum 20Z | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
DVE02_v2_12566 | - |
Methylosinus trichosporium |
HPR | - |
Methylosinus trichosporium |
HPR | - |
Methylotuvimicrobium alcaliphilum |
HPR | - |
Methylococcus capsulatus |
LCv4_3219 | - |
Methylotuvimicrobium alcaliphilum |
MCA1407 | - |
Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Methylosinus trichosporium |
60 | - |
- |
Methylotuvimicrobium alcaliphilum |
60 | - |
- |
Methylococcus capsulatus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
14 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
19 | - |
glyoxylate | cosubstrate NADH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
19 | - |
NADH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
23 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylococcus capsulatus | |
30 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
38 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 37°C | Methylococcus capsulatus | |
41 | - |
glyoxylate | cosubstrate NADH, pH 7, 37°C | Methylococcus capsulatus | |
41 | - |
NADH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylococcus capsulatus | |
42 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 37°C | Methylococcus capsulatus | |
42 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylococcus capsulatus | |
48 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
48 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
52 | - |
glyoxylate | cosubstrate NADPH, pH 7, 30°C | Methylosinus trichosporium | |
52 | - |
NADPH | cosubsubstrate glyoxylate, 30°C, pH 7 | Methylosinus trichosporium | |
88 | - |
glyoxylate | cosubstrate NADPH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
88 | - |
NADPH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
166 | - |
glyoxylate | cosubstrate NADH, pH 7, 30°C | Methylosinus trichosporium | |
166 | - |
NADH | cosubsubstrate glyoxylate, 30°C, pH 7 | Methylosinus trichosporium | |
284 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 | Methylosinus trichosporium | |
288 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 30°C | Methylosinus trichosporium | |
687 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 30°C | Methylosinus trichosporium | |
687 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 | Methylosinus trichosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Methylosinus trichosporium |
7 | - |
- |
Methylotuvimicrobium alcaliphilum |
7 | - |
- |
Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | NADPH is preferred over NADH | Methylotuvimicrobium alcaliphilum | |
NADH | NADH is preferred over NADPH | Methylosinus trichosporium | |
NADH | NADH is preferred over NADPH | Methylococcus capsulatus | |
NADPH | NADPH is preferred over NADH | Methylotuvimicrobium alcaliphilum | |
NADPH | NADH is preferred over NADPH | Methylosinus trichosporium | |
NADPH | NADH is preferred over NADPH | Methylococcus capsulatus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.9 | - |
glyoxylate | cosubstrate NADPH, pH 7, 30°C | Methylosinus trichosporium | |
6 | - |
glyoxylate | cosubstrate NADH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
6 | - |
glyoxylate | cosubstrate NADH, pH 7, 37°C | Methylococcus capsulatus | |
7.5 | - |
glyoxylate | cosubstrate NADH, pH 7, 30°C | Methylosinus trichosporium | |
8 | - |
glyoxylate | cosubstrate NADPH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
10.56 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 37°C | Methylococcus capsulatus | |
50 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
143 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylococcus capsulatus | |
175 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
236 | - |
NADPH | cosubsubstrate glyoxylate, 30°C, pH 7 | Methylosinus trichosporium | |
240 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 30°C | Methylosinus trichosporium | |
271 | - |
NADH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
282 | - |
3-hydroxypyruvate | cosubstrate NADPH, pH 7, 37°C | Methylotuvimicrobium alcaliphilum | |
737 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 37°C | Methylococcus capsulatus | |
873 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
1400 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 37°C, pH 7 | Methylococcus capsulatus | |
2582 | - |
NADPH | cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 | Methylosinus trichosporium | |
2733 | - |
NADH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylococcus capsulatus | |
3142 | - |
NADPH | cosubsubstrate glyoxylate, 37°C, pH 7 | Methylotuvimicrobium alcaliphilum | |
4907 | - |
3-hydroxypyruvate | cosubstrate NADH, pH 7, 30°C | Methylosinus trichosporium | |
12769 | - |
NADH | cosubsubstrate glyoxylate, 30°C, pH 7 | Methylosinus trichosporium | |
24535 | - |
NADH | cosubsubstrate 3-hydroxypyruvate, 30°C, pH 7 | Methylosinus trichosporium |