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Literature summary for 1.1.1.81 extracted from

  • Lassalle, L.; Engilberge, S.; Madern, D.; Vauclare, P.; Franzetti, B.; Girard, E.
    New insights into the mechanism of substrates trafficking in glyoxylate/hydroxypyruvate reductases (2016), Sci. Rep., 6, 20629.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Pyrococcus horikoshii
expressed in Escherichia coli BL21(DE3) cells Pyrococcus yayanosii
expressed in Escherichia coli BL21(DE3) cells Pyrococcus furiosus

Crystallization (Commentary)

Crystallization (Comment) Organism
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl Pyrococcus horikoshii
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl Pyrococcus yayanosii
in complex with D-glycerate and NADPH, hanging drop vapor diffusion method, using 100 mM sodium acetate, pH 5.2, 15% (w/v) polyethylene glycol 400 and 100 mM NaCl Pyrococcus furiosus

Inhibitors

Inhibitors Comment Organism Structure
Hydroxypyruvate
-
Pyrococcus furiosus
Hydroxypyruvate
-
Pyrococcus horikoshii
Hydroxypyruvate
-
Pyrococcus yayanosii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus horikoshii
0.07
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus horikoshii
0.09
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus furiosus
0.14
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus furiosus
0.165
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus yayanosii
0.41
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus yayanosii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxypyruvate + NADH + H+ Pyrococcus horikoshii
-
D-glycerate + NAD+
-
?
hydroxypyruvate + NADH + H+ Pyrococcus yayanosii
-
D-glycerate + NAD+
-
?
hydroxypyruvate + NADH + H+ Pyrococcus furiosus
-
D-glycerate + NAD+
-
?
hydroxypyruvate + NADH + H+ Pyrococcus yayanosii CH1
-
D-glycerate + NAD+
-
?
hydroxypyruvate + NADPH + H+ Pyrococcus horikoshii
-
D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+ Pyrococcus yayanosii
-
D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+ Pyrococcus furiosus
-
D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+ Pyrococcus yayanosii CH1
-
D-glycerate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Pyrococcus furiosus Q8U3Y2
-
-
Pyrococcus horikoshii
-
-
-
Pyrococcus yayanosii F8AEA4
-
-
Pyrococcus yayanosii CH1 F8AEA4
-
-

Purification (Commentary)

Purification (Comment) Organism
Resource Q column chromatography and Superose 12 column chromatography Pyrococcus horikoshii
Resource Q column chromatography and Superose 12 column chromatography Pyrococcus yayanosii
Resource Q column chromatography and Superose 12 column chromatography Pyrococcus furiosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxypyruvate + NADH + H+
-
Pyrococcus horikoshii D-glycerate + NAD+
-
?
hydroxypyruvate + NADH + H+
-
Pyrococcus yayanosii D-glycerate + NAD+
-
?
hydroxypyruvate + NADH + H+
-
Pyrococcus furiosus D-glycerate + NAD+
-
?
hydroxypyruvate + NADH + H+
-
Pyrococcus yayanosii CH1 D-glycerate + NAD+
-
?
hydroxypyruvate + NADPH + H+
-
Pyrococcus horikoshii D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+
-
Pyrococcus yayanosii D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+
-
Pyrococcus furiosus D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+
-
Pyrococcus yayanosii CH1 D-glycerate + NADP+
-
?

Synonyms

Synonyms Comment Organism
glyoxylate/hydroxypyruvate reductase
-
Pyrococcus horikoshii
glyoxylate/hydroxypyruvate reductase
-
Pyrococcus yayanosii
glyoxylate/hydroxypyruvate reductase
-
Pyrococcus furiosus
GRHPR
-
Pyrococcus horikoshii
GRHPR
-
Pyrococcus yayanosii
GRHPR
-
Pyrococcus furiosus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.6
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus horikoshii
1.2
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus furiosus
4.5
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus horikoshii
6.5
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus yayanosii
10
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus furiosus
25
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus yayanosii

Cofactor

Cofactor Comment Organism Structure
NADH the enzyme prefers NADH over NADPH Pyrococcus horikoshii
NADH the enzyme prefers NADH over NADPH Pyrococcus yayanosii
NADH the enzyme prefers NADH over NADPH Pyrococcus furiosus
NADPH the enzyme prefers NADH over NADPH Pyrococcus horikoshii
NADPH the enzyme prefers NADH over NADPH Pyrococcus yayanosii
NADPH the enzyme prefers NADH over NADPH Pyrococcus furiosus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.35
-
Hydroxypyruvate in the presence of NADH, at pH 8.0 and 50°C Pyrococcus furiosus
0.76
-
Hydroxypyruvate in the presence of NADH, at pH 8.0 and 50°C Pyrococcus yayanosii
2
-
Hydroxypyruvate in the presence of NADPH, at pH 8.0 and 50°C Pyrococcus yayanosii
3.82
-
Hydroxypyruvate in the presence of NADH, at pH 8.0 and 50°C Pyrococcus horikoshii

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus furiosus
30
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus horikoshii
40
-
Hydroxypyruvate with NADPH as cosubstrate, at pH 8.0 and 50°C Pyrococcus yayanosii
60
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus yayanosii
65
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus horikoshii
75
-
Hydroxypyruvate with NADH as cosubstrate, at pH 8.0 and 50°C Pyrococcus furiosus