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Literature summary for 1.1.1.81 extracted from

  • Shen, P.H.; Wu, B.
    Over-expression of a hydroxypyruvate reductase in Methylobacterium sp. MB200 enhances glyoxylate accumulation (2007), J. Ind. Microbiol. Biotechnol., 34, 657-663.
    View publication on PubMed

Application

Application Comment Organism
synthesis potential application in the enzymatic synthesis of glyoxylate Methylobacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
expressed as His-tag fusion protein in Escherichia coli Turner (DE3) Methylobacterium sp.
expressed in host strain to create the new strain Methylobacterium sp. MB201, 2fold increase in glyoxylate production Methylobacterium sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34000
-
x * 34000, SDS-PAGE Methylobacterium sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
hydroxypyruvate + NADPH + H+ Methylobacterium sp. key enzyme of the serine cycle D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+ Methylobacterium sp. MB200 key enzyme of the serine cycle D-glycerate + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Methylobacterium sp.
-
capable of producing glyoxylate from methanol
-
Methylobacterium sp. MB200
-
capable of producing glyoxylate from methanol
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tag protein from Escherichia coli Methylobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
hydroxypyruvate + NADPH + H+
-
Methylobacterium sp. D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+ key enzyme of the serine cycle Methylobacterium sp. D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+
-
Methylobacterium sp. MB200 D-glycerate + NADP+
-
?
hydroxypyruvate + NADPH + H+ key enzyme of the serine cycle Methylobacterium sp. MB200 D-glycerate + NADP+
-
?

Subunits

Subunits Comment Organism
? x * 34000, SDS-PAGE Methylobacterium sp.

Synonyms

Synonyms Comment Organism
HRP
-
Methylobacterium sp.

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Methylobacterium sp.