Application | Comment | Organism |
---|---|---|
synthesis | potential application in the enzymatic synthesis of glyoxylate | Methylobacterium sp. |
Cloned (Comment) | Organism |
---|---|
expressed as His-tag fusion protein in Escherichia coli Turner (DE3) | Methylobacterium sp. |
expressed in host strain to create the new strain Methylobacterium sp. MB201, 2fold increase in glyoxylate production | Methylobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34000 | - |
x * 34000, SDS-PAGE | Methylobacterium sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NADPH + H+ | Methylobacterium sp. | key enzyme of the serine cycle | D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | Methylobacterium sp. MB200 | key enzyme of the serine cycle | D-glycerate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylobacterium sp. | - |
capable of producing glyoxylate from methanol | - |
Methylobacterium sp. MB200 | - |
capable of producing glyoxylate from methanol | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tag protein from Escherichia coli | Methylobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NADPH + H+ | - |
Methylobacterium sp. | D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | key enzyme of the serine cycle | Methylobacterium sp. | D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | - |
Methylobacterium sp. MB200 | D-glycerate + NADP+ | - |
? | |
hydroxypyruvate + NADPH + H+ | key enzyme of the serine cycle | Methylobacterium sp. MB200 | D-glycerate + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 34000, SDS-PAGE | Methylobacterium sp. |
Synonyms | Comment | Organism |
---|---|---|
HRP | - |
Methylobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Methylobacterium sp. |