Cloned (Comment) | Organism |
---|---|
expression of His-tagged wild-type and mutant enzymes in Escherichia coli | Homo sapiens |
gene GRHPR, localization of chromosome 9q12, DNA and amino acid sequence determination and analysis, genetic structure and promoter analysis, expression analysis, expression as GFP-fusion protein in the cytosol of HEK293 cells, co-expression with PPARalpha in HepG2 cells and regulation, overview | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
purified detagged recombinant enzyme in ternary complex with product D-glycerate and cofactor NADPH, sitting drop vapour diffusion method, 5.5 mg/ml protein in 20 mM Tris-HCl, pH 8.5, 1 mM 2-mercaptoethanol, 0.2 mM NADPH, and 0.5 mm di-sodium oxalate, mixed with mother liquor, containing 15% w/v PEG 8000, 0.2 M ammonium sulfate, and 0.1 M sodium cacodylate, pH 6.5, to 0.002 ml drops, 18°C, X-ray diffraction structure determination and analysis at 2.2 A resolution | Mus musculus |
purified detagged recombinant enzyme in ternary complex with product D-glycerate and cofactor NADPH, sitting drop vapour diffusion method, 5.5 mg/ml protein in 20 mM Tris-HCl, pH 8.5, 1 mM 2-mercaptoethanol, 0.2 mM NADPH, and 0.5 mm di-sodium oxalate, mixed with mother liquor, containing 15% w/v PEG 8000, 0.2 M ammonium sulfate, and 0.1 M sodium cacodylate, pH 6.5, to 0.002 ml drops, 18°C, X-ray diffraction structure determination and analysis at 2.2 A resolution | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
G160R | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Mus musculus |
G160R | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Homo sapiens |
G165D | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Mus musculus |
G165D | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Homo sapiens |
M322R | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Mus musculus |
M322R | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Homo sapiens |
R302C | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Mus musculus |
R302C | site-directed mutagenesis, the mutant shows reduced catalytic activity compared to the wild-type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-glycerate | the enzyme shows product inhibition | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Mus musculus | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | Mus musculus | - |
glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | Homo sapiens | the enzyme is involved in removal of the metabolic by-product from liver | glycolate + NAD(P)+ | - |
? | |
hydroxypyruvate + NAD(P)H | Mus musculus | - |
D-glycerate + NAD(P)+ + H+ | - |
? | |
hydroxypyruvate + NAD(P)H | Homo sapiens | - |
D-glycerate + NAD(P)+ + H+ | - |
? | |
additional information | Homo sapiens | enzyme deficiency leads to primary hyperoxaluria type 2 with increased urinary oxalate levels, formation of kidney stones, and renal failure | ? | - |
? | |
additional information | Mus musculus | the enzyme is transcriptionally regulated by the peroxisome proliferator-activated receptor alpha, PPARalpha, in liver, overview | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Mus musculus | - |
gene GRHPR | - |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, the His-tag is cleaved by thrombin followed by gel filtration, over 95% purity | Mus musculus |
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli by nickel affinity chromatography, the His-tag is cleaved by thrombin followed by gel filtration, over 95% purity | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Mus musculus | - |
liver | - |
Mus musculus | - |
liver | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glyoxylate + NAD(P)H | - |
Mus musculus | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | - |
Homo sapiens | glycolate + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | the enzyme is involved in removal of the metabolic by-product from liver | Homo sapiens | glycolate + NAD(P)+ | - |
? | |
hydroxypyruvate + NAD(P)H | - |
Mus musculus | D-glycerate + NAD(P)+ + H+ | - |
? | |
hydroxypyruvate + NAD(P)H | - |
Homo sapiens | D-glycerate + NAD(P)+ + H+ | - |
? | |
additional information | enzyme deficiency leads to primary hyperoxaluria type 2 with increased urinary oxalate levels, formation of kidney stones, and renal failure | Homo sapiens | ? | - |
? | |
additional information | the enzyme is transcriptionally regulated by the peroxisome proliferator-activated receptor alpha, PPARalpha, in liver, overview | Mus musculus | ? | - |
? | |
additional information | structural basis of enzyme substrate specificity, active site structure and substrate binding, no activity with pyruvate, overview | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
D-2-hydroxy-acid dehydrogenase | - |
Homo sapiens |
glyoxylate reductase/hydroxypyruvate reductase | - |
Mus musculus |
glyoxylate reductase/hydroxypyruvate reductase | - |
Homo sapiens |
GRHPR | - |
Mus musculus |
GRHPR | - |
Homo sapiens |
More | the enzyme shows bifunctionality also performing the reaction of hydroxypyruvate reductase, EC 1.1.1.81 | Mus musculus |
More | the enzyme shows bifunctionality also performing the reaction of hydroxypyruvate reductase, EC 1.1.1.81 | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Mus musculus | |
NADH | - |
Homo sapiens | |
NADPH | - |
Mus musculus | |
NADPH | binding structure | Homo sapiens |