General Stability | Organism |
---|---|
activity is slowly lost on storage at -15°C interspersed with frequent thawing and re-freezing, about 20% activity is lost over 8 cycles of freezing and thawing | Paracoccus denitrificans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bromide | 33 mM, 38% inhibition | Paracoccus denitrificans | |
citrate | competitive vs. hydroxypyruvate | Paracoccus denitrificans | |
Iodide | 33 mM, 39% inhibition | Paracoccus denitrificans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
hydroxypyruvate + NAD(P)H | Paracoccus denitrificans | - |
D-glycerate + NAD(P)+ + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paracoccus denitrificans | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.077 | - |
oxidation of glycerate | Paracoccus denitrificans |
0.135 | - |
cofactor NADH | Paracoccus denitrificans |
0.33 | - |
cofactor NADPH | Paracoccus denitrificans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,3-butandione + NAD(P)H | 33 mM, 12% of activity with hydroxypyruvate | Paracoccus denitrificans | butan-2-ol-3-one + NAD(P)+ | - |
? | |
acetoin + NAD(P)H + H+ | 33 mM, 14% of activity with hydroxypyruvate | Paracoccus denitrificans | 2,3-butanediol + NAD(P)+ | - |
? | |
glyoxylate + NAD(P)H | 33 mM, 15% activity | Paracoccus denitrificans | glycolate + NAD(P)+ | - |
? | |
hydroxypyruvate + NAD(P)H | - |
Paracoccus denitrificans | D-glycerate + NAD(P)+ + H+ | - |
? | |
hydroxypyruvate + NAD(P)H | - |
Paracoccus denitrificans | D-glycerate + NAD(P)+ + H+ | - |
r | |
oxaloacetate + NAD(P)H | 33 mM, 32% of activity with hydroxypyruvate | Paracoccus denitrificans | malate + NAD(P)+ | - |
? |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
42 | - |
half-life of 35 min in 50 mM phosphate buffer, pH 7.0 | Paracoccus denitrificans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
sharp peak | Paracoccus denitrificans |