Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | competitive activator with respect to dihydroxyacetone phosphate above 30 mM | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | effective inhibitor | Homo sapiens | |
iodoacetate | 50 mM, incubated for 60 min | Homo sapiens | |
NADPH | - |
Homo sapiens | |
p-chloromercuribenzoate | effective inhibitor | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
35000 | - |
2 * 35000, SDS-PAGE | Homo sapiens |
74000 | - |
electrophoresis in a non-denaturing system | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | Homo sapiens | - |
dihydroxyacetone phosphate + NADH + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
affinity chromatography | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
adipose tissue | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
250 | - |
- |
Homo sapiens |
Storage Stability | Organism |
---|---|
-20°C, 50% glycerol, retains activity for several months | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
sn-glycerol 3-phosphate + NAD+ | - |
Homo sapiens | dihydroxyacetone phosphate + NADH + H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 35000, SDS-PAGE | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
dihydroxyacetone phosphate reduction | Homo sapiens |
9 | - |
glycerol-3-phosphate oxidation | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADPH | Homo sapiens | |
NAD+ | - |
Homo sapiens | |
NADH | - |
Homo sapiens |